Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process
| Autor: | Jan Sklenar, Yasin F. Dagdas, Silke Robatzek, Sophien Kamoun, Joe Win, Rosa Lozano-Durán, Jack H. Vossen, Benjamin Petre, Mauricio P Contreras, Alexandra M. E. Jones, Ahmed Abd-El-Haliem, Sebastian Schornack, Frank L.H. Menke, Roger Castells-Graells, Tolga O. Bozkurt, Martin H. Schattat |
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| Přispěvatelé: | The Sainsbury Laboratory [Norwich] (TSL), Biotechnology and Biological Sciences Research Council (BBSRC) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine [SDV]Life Sciences [q-bio] Plant Biology Nicotiana benthamiana Plant Science 0601 Biochemistry and Cell Biology 01 natural sciences Interactome 2.1 Biological and endogenous factors Protein Interaction Maps Aetiology Pathogen Plant Proteins 0303 health sciences Effector food and beverages Cell biology Infectious Diseases Host-Pathogen Interactions Phytophthora infestans Infection SNARE Proteins Endosome Immunoprecipitation Large-Scale Biology Articles Plant Biology & Botany 0607 Plant Biology Endosomes Biology 03 medical and health sciences Tobacco Genetics Life Science Transport Vesicles SB 030304 developmental biology Plant Diseases 0604 Genetics Host (biology) Cell Membrane fungi QK Proteins Cell Biology biology.organism_classification QR Plant Breeding 030104 developmental biology Biochemistry and Cell Biology Function (biology) 010606 plant biology & botany |
| Zdroj: | Plant Cell The Plant cell, vol 33, iss 5 Plant Cell, 33(5), 1447-1471 Plant Cell 33 (2021) 5 |
| ISSN: | 1040-4651 |
| Popis: | Pathogens modulate plant cell structure and function by secreting effectors into host tissues. Effectors typically function by associating with host molecules and modulating their activities. This study aimed to identify the host processes targeted by the RXLR class of host-translocated effectors of the potato blight pathogen Phytophthora infestans. To this end, we performed an in planta protein-protein interaction screen by transiently expressing P. infestans RXLR effectors in Nicotiana benthamiana leaves followed by co-immunoprecipitation (co-IP) and liquid chromatography tandem mass spectrometry (LC-MS/MS). This screen generated an effector-host protein interactome matrix of 59 P. infestans RXLR effectors x 586 N. benthamiana proteins. Classification of the host interactors into putative functional categories revealed over 35 biological processes possibly targeted by P. infestans. We further characterized the PexRD12/31 family of RXLR-WY effectors, which associate and co-localize with components of the vesicle trafficking machinery. One member of this family, PexRD31, increased the number of FYVE positive vesicles in N. benthamiana cells. FYVE positive vesicles also accumulated in leaf cells near P. infestans hyphae, indicating that the pathogen may enhance endosomal trafficking during infection. We anticipate that the interactome dataset we generated will serve as a useful community resource for functional studies of P. infestans effectors and of effector-targeted host processes. |
| Databáze: | OpenAIRE |
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