Responses of the chloroplast glyoxalase system to high CO2 concentrations
| Autor: | Chikahiro Miyake, Amane Makino, Yuji Suzuki, Hiroshi Fukayama, Kimitsune Ishizaki, Katsuhiko Sakamoto, Akiko Nishi, Kentaro Ifuku, Ginga Shimakawa, Ryutaro Morita |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Carbohydrate metabolism Photosynthesis Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound methylglyoxal Arabidopsis thaliana Molecular Biology photosynthesis Phototroph biology Chemistry Organic Chemistry Methylglyoxal food and beverages glyoxalase system General Medicine Dicarbonyls biology.organism_classification Chloroplast Cytosol 030104 developmental biology high [CO2] Biotechnology Glyoxalase system |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 82(12):2072-2083 |
| ISSN: | 0916-8451 |
| Popis: | Sugar metabolism pathways such as photosynthesis produce dicarbonyls, e.g. methylglyoxal (MG), which can cause cellular damage. The glyoxalase (GLX) system comprises two enzymes GLX1 and GLX2, and detoxifies MG; however, this system is poorly understood in the chloroplast, compared with the cytosol. In the present study, we determined GLX1 and GLX2 activities in spinach chloroplasts, which constituted 40% and 10%, respectively, of the total leaf glyoxalase activity. In Arabidopsis thaliana, five GFP-fusion GLXs were present in the chloroplasts. Under high CO2 concentrations, where increased photosynthesis promotes the MG production, GLX1 and GLX2 activities in A. thaliana increased and the expression of AtGLX1-2 and AtGLX2-5 was enhanced. On the basis of these findings and the phylogeny of GLX in oxygenic phototrophs, we propose that the GLX system scavenges MG produced in chloroplasts during photosynthesis. |
| Databáze: | OpenAIRE |
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