Neuroscience in the third dimension: shedding new light on the brain with tissue clearing
| Autor: | Alain Chédotal, Morgane Belle, Robin J. Vigouroux |
|---|---|
| Přispěvatelé: | Institut de la Vision, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), BMC, BMC |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
[SDV]Life Sciences [q-bio] Review Fluorescence Field (computer science) lcsh:RC346-429 law.invention 03 medical and health sciences Cellular and Molecular Neuroscience law Research community Clearing Animals Humans Orchestration (computing) Dimension (data warehouse) Molecular Biology lcsh:Neurology. Diseases of the nervous system Protocol (science) Clarity Tissue clearing Tissue-clearing Neurosciences Brain 3DISCO [SDV] Life Sciences [q-bio] 030104 developmental biology CLARITY RNA Psychology Neuroscience iDISCO |
| Zdroj: | Molecular Brain, Vol 10, Iss 1, Pp 1-10 (2017) Molecular Brain Molecular Brain, BioMed Central, 2017, 10 (1), pp.33. ⟨10.1186/s13041-017-0314-y⟩ Molecular Brain, 2017, 10 (1), pp.33. ⟨10.1186/s13041-017-0314-y⟩ |
| ISSN: | 1756-6606 |
| DOI: | 10.1186/s13041-017-0314-y |
| Popis: | International audience; AbstractFor centuries analyses of tissues have depended on sectioning methods. Recent developments of tissue clearing techniques have now opened a segway from studying tissues in 2 dimensions to 3 dimensions. This particular advantage echoes heavily in the field of neuroscience, where in the last several years there has been an active shift towards understanding the complex orchestration of neural circuits. In the past five years, many tissue-clearing protocols have spawned. This is due to varying strength of each clearing protocol to specific applications. However, two main protocols have shown their applicability to a vast number of applications and thus are exponentially being used by a growing number of laboratories. In this review, we focus specifically on two major tissue-clearing method families, derived from the 3DISCO and the CLARITY clearing protocols. Moreover, we provide a “hands-on” description of each tissue clearing protocol and the steps to look out for when deciding to choose a specific tissue clearing protocol. Lastly, we provide perspectives for the development of tissue clearing protocols into the research community in the fields of embryology and cancer. |
| Databáze: | OpenAIRE |
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