Epstein-Barr virus genotypes in NPC biopsies from north Africa
| Autor: | J. Abdelwahab, J. Dubreuil, Abdelmadjide Bouguermouh, D. Ellouz, G. Decaussin, M. Bouzid, Tadamasa Ooka, D. Djennaoui |
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| Rok vydání: | 1994 |
| Předmět: |
Cancer Research
China Herpesvirus 4 Human Tunisia Genotype Biopsy medicine.disease_cause Polymerase Chain Reaction Virus Cell Line hemic and lymphatic diseases otorhinolaryngologic diseases medicine Gammaherpesvirinae Humans Saliva biology Deoxyribonuclease BamHI Nasopharyngeal Neoplasms biology.organism_classification medicine.disease Virology Epstein–Barr virus stomatognathic diseases Restriction site Restriction enzyme Blotting Southern Oncology Nasopharyngeal carcinoma Nasopharyngitis Algeria Polymorphism Restriction Fragment Length |
| Zdroj: | International journal of cancer. 56(4) |
| ISSN: | 0020-7136 |
| Popis: | The genotypes of Epstein-Barr virus (EBV) were investigated in North African nasopharyngeal carcinoma (NPC) biopsies, nasopharyngeal chronic inflammation (NCI) biopsies, and saliva of healthy individuals from Algeria and Tunisia where there is an intermediate incidence of NPC. The prevalence of A-type virus in NPC, NCI biopsies and saliva of healthy individuals was found in these regions by means of a PCR assay. Restriction enzyme polymorphism analysis by Southern blotting revealed that all North African EBV variants have a conserved restriction site on BamHI W'-I' and XhoI LMP gene. No additional BamHI enzyme site on the BamHI-F fragment was observed; however, the presence of an extra BamHI site on the BamHI-H fragment giving 2 HI and H2 fragment-like EBV M-ABA strains was found. All EBV strains present in NPC or NCI biopsies at all ages were homogeneous in these polymorphisms and no correlation was observed between the EBV genotypes from NPC patients and clinical stages of the cancer. These characteristics revealed a significant difference between the EBV variants common in Chinese NPC and those in North African NPC. |
| Databáze: | OpenAIRE |
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