Pharmacology of captan: Biochemical effects with special reference to macromolecular synthesis
| Autor: | Loretta M. Atkins, Walker Em, Gale Gr, Richard H. Gadsden, Alayne B. Smith |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Antifungal Agents
Guanine Indoles Macromolecular Substances In Vitro Techniques Biology Tritium Toxicology chemistry.chemical_compound Leucine In vivo Animals Cysteine RNA Neoplasm Carcinoma Ehrlich Tumor Purine metabolism Uridine Captan Candida Pharmacology Carbon Isotopes Adenine food and beverages RNA Serum Albumin Bovine DNA Neoplasm In vitro Neoplasm Proteins Biochemistry chemistry Depression Chemical Thymidine Drug Antagonism |
| Zdroj: | Toxicology and Applied Pharmacology. 18:426-441 |
| ISSN: | 0041-008X |
| DOI: | 10.1016/0041-008x(71)90135-9 |
| Popis: | Captan ( N -[trichloromethylthio]-4-cyclohexene-1,2-dicarboximide), a widely used agricultural fungicide, is a potent inhibitor of incorporation of isotopically labeled thymidine, uridine, and l -leucine into the acid-insoluble fraction of Ehrlich ascites tumor cells in vitro . The inhibitory actions of quite low concentrations can be annulled partially by washing the cells with fresh medium after exposure; the actions of higher concentrations are not reversible under these conditions. Prior addition to the medium of thiol compounds or albumin protects the cells from the actions of the inhibitor. A substantial portion of captan is bound to or otherwise inactivated by the cells. Degradation of DNA and RNA to an acid-soluble state also occurs upon exposure of the cells to the fungicide. Glycolysis is highly sensitive to captan in vitro , and this effect is likewise antagonized by thiol compounds. Captan inhibits the synthesis of RNA purines- 14 C from formate- 14 C in ascites tumor cells in vivo as well as in cultures of Candida albicans ; this action on tumor cells was demonstrated after intraperitoneal or oral administration of the inhibitor. The profound depression by captan of the rate of development of the ascites tumor in vivo reported by other workers could not be confirmed. |
| Databáze: | OpenAIRE |
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