Interplay between human microglia and neural stem/progenitor cells in an allogeneic co‐culture model

Autor: Elisabet Åkesson, Jia Liu, Marianne Schultzberg, Eva-Britt Samuelsson, Mingqin Zhu, Erik Hjorth, Cinzia Calzarossa
Rok vydání: 2013
Předmět:
Cell Survival
Cellular differentiation
microglia
Receptors
Cell Surface
Cell Communication
Biology
Regenerative Medicine
immunomodulation
M1/M2 phenotype
Transforming Growth Factor beta1
03 medical and health sciences
0302 clinical medicine
Neural Stem Cells
Phagocytosis
Antigens
CD
Orexin Receptors
otorhinolaryngologic diseases
medicine
Humans
Progenitor cell
Neural cell
Cells
Cultured
Cell Proliferation
030304 developmental biology
0303 health sciences
Microglia
Glial fibrillary acidic protein
Interleukin-6
Cell Differentiation
transforming growth factor-β
Original Articles
Cell Biology
Nestin
Allografts
Coculture Techniques
Neural stem cell
Cell biology
stomatognathic diseases
Phenotype
medicine.anatomical_structure
CD200
Antigens
Surface
biology.protein
Molecular Medicine
Neural cell adhesion molecule
030217 neurology & neurosurgery
human neural stem/progenitor cells
Zdroj: Journal of Cellular and Molecular Medicine
ISSN: 1582-4934
1582-1838
DOI: 10.1111/jcmm.12123
Popis: Experimental neural cell therapies, including donor neural stem/progenitor cells (NPCs) have been reported to offer beneficial effects on the recovery after an injury and to counteract inflammatory and degenerative processes in the central nervous system (CNS). The interplay between donor neural cells and the host CNS still to a large degree remains unclear, in particular in human allogeneic conditions. Here, we focused our studies on the interaction of human NPCs and microglia utilizing a co-culture model. In co-cultures, both NPCs and microglia showed increased survival and proliferation compared with mono-cultures. In the presence of microglia, a larger subpopulation of NPCs expressed the progenitor cell marker nestin, whereas a smaller group of NPCs expressed the neural markers polysialylated neural cell adhesion molecule, A2B5 and glial fibrillary acidic protein compared with NPC mono-cultures. Microglia thus hindered differentiation of NPCs. The presence of human NPCs increased microglial phagocytosis of latex beads. Furthermore, we observed that the expression of CD200 molecules on NPCs and the CD200 receptor protein on microglia was enhanced in co-cultures, whereas the release of transforming growth factor-β was increased suggesting anti-inflammatory features of the co-cultures. To conclude, the interplay between human allogeneic NPCs and microglia, significantly affected their respective proliferation and phenotype. Neural cell therapy including human donor NPCs may in addition to offering cell replacement, modulate host microglial phenotypes and functions to benefit neuroprotection and repair.
Databáze: OpenAIRE
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