In Vitro–expanded Antigen-specific Regulatory T Cells Suppress Autoimmune Diabetes
| Autor: | Greg Szot, Jianqin Ye, Jeffrey A. Bluestone, Qizhi Tang, Mingying Bi, Kammi J. Henriksen, Mark Bonyhadi, Erik B. Finger, Hugh O. McDevitt, Emma L. Masteller |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Interleukin 2
Adoptive cell transfer immunoregulation T cell Immunology Receptors Antigen T-Cell Mice Transgenic chemical and pharmacologic phenomena Biology medicine.disease_cause Autoantigens T-Lymphocytes Regulatory Article Autoimmunity Mice 03 medical and health sciences 0302 clinical medicine Antigen Mice Inbred NOD CD4+CD25+ T cells medicine Animals Immunology and Allergy IL-2 receptor 030304 developmental biology NOD mice Mice Inbred BALB C 0303 health sciences tolerance autoimmunity Receptors Interleukin-2 hemic and immune systems medicine.disease Adoptive Transfer 3. Good health Transplant rejection Diabetes Mellitus Type 1 medicine.anatomical_structure CD4 Antigens 030215 immunology medicine.drug |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| Popis: | The low number of CD4+ CD25+ regulatory T cells (Tregs), their anergic phenotype, and diverse antigen specificity present major challenges to harnessing this potent tolerogenic population to treat autoimmunity and transplant rejection. In this study, we describe a robust method to expand antigen-specific Tregs from autoimmune-prone nonobese diabetic mice. Purified CD4+ CD25+ Tregs were expanded up to 200-fold in less than 2 wk in vitro using a combination of anti-CD3, anti-CD28, and interleukin 2. The expanded Tregs express a classical cell surface phenotype and function both in vitro and in vivo to suppress effector T cell functions. Most significantly, small numbers of antigen-specific Tregs can reverse diabetes after disease onset, suggesting a novel approach to cellular immunotherapy for autoimmunity. |
| Databáze: | OpenAIRE |
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