Heat stress modulates polymorphonuclear cell response in early pregnancy cows: I. interferon pathway and oxidative stress
| Autor: | Alfredo Q. Antoniazzi, Lady Katerine Serrano Mujica, Carolina dos Santos Amaral, Suzan Gonçalves Rosa, Cristina W. Nogueira, Valério M. Portela, William Schoenau, M. F. Fiorenza, Natalia P. Smirnova, Fabio V. Comim, Gabrielle Rebeca Everling Correa |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Myxovirus Resistance Proteins
Neutrophils Physiology Gene Expression medicine.disease_cause Biochemistry chemistry.chemical_compound White Blood Cells Cell Signaling Interferon Animal Cells Pregnancy Malondialdehyde Gene expression Medicine and Health Sciences Lipid Hormones Progesterone Insemination Artificial Ultrasonography Multidisciplinary Physics Temperature Classical Mechanics Signaling Cascades Body Fluids medicine.anatomical_structure Blood Physical Sciences Mechanical Stress Cytokines Medicine Female Cellular Types Anatomy Corpus luteum medicine.drug Research Article Signal Transduction Immune Cells Science Immunology Biology Stress Signaling Cascade Andrology Corpus Luteum medicine Genetics Animals Estrous cycle Blood Cells Biology and Life Sciences Proteins Cell Biology medicine.disease Hormones Interferon tau Oxidative Stress Thermal Stresses chemistry Cattle Interferons Oxidative stress Heat-Shock Response |
| Zdroj: | PLoS ONE, Vol 16, Iss 9, p e0257418 (2021) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | One of the major causes of early pregnancy loss is heat stress. In ruminants, interferon tau (IFNT) is the embryo signal to the mother. Once the interferon signaling pathway is activated, it drives gene expression for interferon-stimulated genes (ISGs) and alters neutrophils responses. The aim of the present study was to evaluate interferon (IFN) pathway, ISGs and gene expression in polymorphonuclear leukocytes (PMN) and oxidative stress in dairy cows under heat stress. Pregnant cows had their estrous cycle synchronized and randomly assigned to a comfort or heat stress group. Blood samples were collected at artificial insemination (AI) and on Days 10, 14 and 18 following AI. Pregnant cows were pregnancy checked by ultrasound on Day 30 and confirmed on Day 60 post-AI. Results are presented as mean ± SEM. The corpus luteum (CL) diameter was not different between groups of pregnant cows; concentration of progesterone of pregnant cows on Day 18 following AI was greater in comfort group compared to heat stressed group. Comfort pregnant cows had higher expression of all analyzed genes from interferon pathway, except for IFNAR1, on both Days 14 and 18. Conversely, heat stressed cows did not show altered expression of IFNT pathway genes and ISGs between Days 10, 14, and 18 after AI. The oxidative stress, determined as malondialdehyde (MDA) levels, was greater in heat stress group on Days 10, 14 and 18, independent of pregnancy status. Heat stress negatively influences expression of ISGs, IFN pathway gene expression in neutrophils, and oxidative stress. Our data suggest that lower conception rates in cows under heat stress are multifactorial, with the association of interferon pathway activation and the unbalanced oxidative stress being main contributing factors. |
| Databáze: | OpenAIRE |
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