Intramammary immunization with live-attenuated Staphylococcus aureus: microbiological and immunological studies in a mouse mastitis model
| Autor: | M. Magdalena Gherardi, Daniel O. Sordelli, Norberto Sanjuan, M. Cristina Cerquetti, Marisa Ines Gomez, Verónica E. García, Mercedes F. Iglesias |
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| Rok vydání: | 1996 |
| Předmět: |
Microbiology (medical)
Immunoglobulin A Staphylococcus aureus Immunology Immunization Secondary Mastitis medicine.disease_cause Vaccines Attenuated Microbiology Immunoglobulin G Mice Mammary Glands Animal Immunity medicine Immunology and Allergy Animals Colony-forming unit biology business.industry Vaccination General Medicine Staphylococcal Infections medicine.disease Intramammary route Infectious Diseases Milk biology.protein Female business |
| Zdroj: | FEMS immunology and medical microbiology. 14(1) |
| ISSN: | 0928-8244 |
| Popis: | Mammary infection was induced in lactating mice by intramammary injection of Staphylococcus aureus. Histopathological analysis revealed infiltration and lesions of varying magnitude that were still apparent 21 days after the challenge. Concomitantly, viable S. aureus was recovered from infected mammary glands. Mice were immunized by the intramammary route with 5 × 106 colony forming units of a temperature-sensitive mutant of S. aureus and subsequently received a boosting injection seven days later. On day 14 mice were challenged by the intramammary route with the wild-type strain. Intramammary immunization induced a significant increase in milk IgA (P < 0.05), serum IgG (P < 0.05) and serum IgA (P < 0.05) on the day of the challenge, when compared with non-immunized mice. Immunization decreased significantly (P < 0.01) the number of S. aureus colony forming units recovered 96 h after intramammary challenge. In conclusion, the feasibility of immunizing locally with temperature-sensitive S. aureus to induce immunity in the mouse mammary gland was demonstrated. The mouse model of mastitis is proposed as a useful system for screening temperature-sensitive S. aureus strains to be utilized in the development of a vaccine. |
| Databáze: | OpenAIRE |
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