Invasiveness of the Yersinia pestis ail protein contributes to host dissemination in pneumonic and oral plague
| Autor: | Hongxiang Chen, Yingmiao Zhang, John D. Klena, Lingyu Jiang, Huahua Cai, Yingxia He, Xiaoling Ying, Gregory V. Plano, Sara Schesser Bartra, Song Zhang, Tie Chen, Mikael Skurnik |
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| Přispěvatelé: | Helsinki One Health (HOH), Research Programs Unit, Mikael Skurnik / Principal Investigator, HUSLAB, Department of Bacteriology and Immunology, HUMI - Human Microbiome Research, Faculty of Medicine, University of Helsinki, Helsinki University Hospital Area |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pneumonic plague Virulence Factors Yersinia pestis 030106 microbiology Mutant YOP DELIVERY Virulence Oral plague EUKARYOTIC CELLS Microbiology Pathogenesis Mice 03 medical and health sciences Invasion Bacterial Proteins medicine Animals PLASMINOGEN-ACTIVATOR PLA Lung Ail protein Host dissemination 11832 Microbiology and virology Mouth Plague biology PSEUDOTUBERCULOSIS BACTERIAL ATTACHMENT MOUSE MODEL IN-VITRO biology.organism_classification medicine.disease Mice Inbred C57BL Disease Models Animal 030104 developmental biology Infectious Diseases Septicemic plague ENTEROCOLITICA 3111 Biomedicine Bacterial outer membrane RESISTANCE Bacteria Bacterial Outer Membrane Proteins |
| Zdroj: | Microbial Pathogenesis. 141:103993 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/j.micpath.2020.103993 |
| Popis: | Yersinia pestis, a Gram-negative bacterium, is the etiologic agent of plague. A hallmark of Y. pestis infection is the organism's ability to rapidly disseminate through an animal host. Y. pestis expresses the outer membrane protein, Ail (Attachment invasion locus), which is associated with host invasion and serum resistance. However, whether Ail plays a role in host dissemination remains unclear. In this study, C57BL/6J mice were challenged with a defined Y. pestis strain, KimD27, or an isogenic ail-deleted mutant derived from KimD27 via metacarpal paw pad inoculation, nasal drops, orogastric infection, or tail vein injection to mimic bubonic, pneumonic, oral, or septicemic plague, respectively. Our results showed that ail-deleted Y. pestis KimD27 lost the ability to invade host cells, leading to failed host dissemination in the pneumonic and oral plague models but not in the bubonic or septicemic plague models, which do not require invasiveness. Therefore, this study demonstrated that whether Ail plays a role in Y. pestis pathogenesis depends on the infection route. |
| Databáze: | OpenAIRE |
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