Capturing in Vivo Plant Metabolism by Real-Time Analysis of Low to High Molecular Weight Volatiles
| Autor: | Alfredo J. Ibáñez, Diego García-Gómez, César Barrios-Collado, Pablo Martinez-Lozano Sinues, Guillermo Vidal-de-Miguel, Renato Zenobi |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Spectrometry Mass Electrospray Ionization Time Factors Light Electrospray ionization Kinetics Analytical chemistry Begoniaceae Tandem mass spectrometry Orbitrap Mass spectrometry 01 natural sciences Analytical Chemistry law.invention 03 medical and health sciences law In vivo Plant metabolism Volatile Organic Compounds Chromatography Chemistry 010401 analytical chemistry 0104 chemical sciences Molecular Weight 030104 developmental biology Gas chromatography |
| Zdroj: | ANALYTICAL CHEMISTRY |
| ISSN: | 1520-6882 |
| Popis: | We have deployed an efficient secondary electrospray ionization source coupled to an Orbitrap mass analyzer (SESI-MS) to investigate the emissions of a Begonia semperflorens. We document how hundreds of species can be tracked with an unparalleled time resolution of 2 min during day-night cycles. To further illustrate the capabilities of this system for volatile organic compounds (VOCs) analysis, we subjected the plant to mechanical damage and monitored its response. As a result, ∼1200 VOCs were monitored displaying different kinetics. To validate the soundness of our in vivo measurements, we fully characterized some key compounds via tandem mass spectrometry (MS/MS) and confirmed their expected behavior based on prior gas chromatography/mass spectrometry (GC/MS) studies. For example, β-caryophyllene, which is directly related to photosynthesis, was found to show a periodic day-night pattern with highest concentrations during the day. We conclude that the capability of SESI-MS to capture highly dynamic VOC emissions and wide analyte coverage makes it an attractive tool to complement GC/MS in plant studies. |
| Databáze: | OpenAIRE |
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