Genetic regulation of the impaired immune response to hepatitis-B vaccine associated with low TCR density in end stage renal disease patients: contribution of complement C4 and factor B alleles
| Autor: | Eszter Ujhelyi, Wladyslaw Sulowicz, Claudia Barth, Veronika Tarján, Józef Stachowski, Conrad A. Baldamus, George Füst, Judit Kramer |
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| Rok vydání: | 1997 |
| Předmět: |
CD4-Positive T-Lymphocytes
HBsAg CD3 Complex CD3 Immunology Receptors Antigen T-Cell Biology Complement factor B Neopterin End stage renal disease Major Histocompatibility Complex Immune system Antigen Renal Dialysis Immunology and Allergy Humans Hepatitis B Vaccines Hepatitis B Antibodies Receptor Alleles Hepatitis B Surface Antigens T-cell receptor Complement C4 biology.protein Kidney Failure Chronic Cell Division Complement Factor B |
| Zdroj: | Immunology letters. 59(1) |
| ISSN: | 0165-2478 |
| Popis: | We have studied the relationship between T-cell receptor (TCR) density, genetic factors and the specific immune response in 153 end stage renal disease (ESRD) patients on haemodialysis immunised with HBsAg vaccine. One-hundred and nineteen patients raised a protective (> 10 U/ml) antibody response to hepatitis-B vaccination (responder, R), while 34 patients were found to be non-responders (NR). The density of the T-cell receptors was determined by flow cytometry. Proliferation of the T-cells induced by autologous monocytes presenting HBsAg was also measured and expressed as a stimulation index (SI). MHC class I, II and III alleles of the patients were also determined. The densities of TCR/CD3 receptors in NR patients were found to be significantly decreased as compared to the R patients (189 +/- 22 vs. 282 +/- 58 arbitrary units, P = 1.3 x 10(-7). TCR/CD3 receptor densities were found to be strongly associated (Spearman correlation coefficient: 0.84, P < 0.000001) with the SI values. Both parameters were found to be under dual genetic control: (a) very low density of the TCR/CD3 receptors and very low SI were found mainly in NR patients carrying HLA-A1, HLA-B8 and HLA-DR3 alleles; and (b) TCR/CD3 densities and function in R group were found to be significantly lower in carriers than in non-carriers of two MHC class III complement protein alleles: C4A*6, and Bf*F. Non-responsiveness to hepatitis-B vaccination was found to be associated with extremely increased neopterin levels. These findings indicate that both genetic and acquired factors contribute to the hepatitis-B vaccination failure in ESRD patients. |
| Databáze: | OpenAIRE |
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