Ultrasound-assisted extraction optimization and validation of ultra-performance liquid chromatographic method for the quantification of miquelianin in Cuphea glutinosa leaves
| Autor: | Amélia T. Henriques, Andreas Sebastian Loureiro Mendez, Mariana Koetz, Marí Castro Santos |
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| Rok vydání: | 2020 |
| Předmět: |
Chromatography
Central composite design Chemistry 010401 analytical chemistry Extraction (chemistry) Fractional factorial design 02 engineering and technology Repeatability 021001 nanoscience & nanotechnology Ultrasound assisted 01 natural sciences 0104 chemical sciences Analytical Chemistry Solvent Plant Leaves chemistry.chemical_compound Glucosides Ultrasonic Waves Cuphea glutinosa Quercetin 0210 nano-technology Miquelianin Chromatography High Pressure Liquid Cuphea |
| Zdroj: | Talanta. 216 |
| ISSN: | 1873-3573 |
| Popis: | Cuphea glutinosa is a medicinal species abundant in South of Brazil, known because of its flavonoids, which have pharmacological properties as antioxidant, anti-hypertensive, diuretic, and antimicrobial. The present study aimed to optimize the extraction and validate an ultra-performance liquid chromatographic method coupled to a photodiode array detector (UPLC-PDA) method for the quantification of a chemical marker miquelianin in C. glutinosa leaves. The optimum conditions for the extraction of miquelianin from leaves of C. glutinosa were determined using a fractional factorial design (FFD) and a central composite design (CCD). An UPLC-PDA method was validated, following the ICH guidelines and RDC 166/2017 of ANVISA (Brazil). The extraction-optimization methodology was obtained with the following parameters: plant:solvent 1:60 (w/v), percentage solvent 38% ethanol, 60 min time, five extractions and particle size ≤ 180 μm. The validation parameters of the quantification method were satisfactory. The results revealed a method with excellent selectivity, linearity, precision (repeatability and intermediate precision were below 2.18 and 1.40%, respectively) and accuracy (mean recovery 90.6%). The average content of miquelianin was 1.03%. Briefly, the optimization of the extractive method in the leaves of C. glutinosa increased the concentration of miquelianin in the crude extract and the method was validated according to the current legislation. |
| Databáze: | OpenAIRE |
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