TBP2 is essential for germ cell development by regulating transcription and chromatin condensation in the oocyte
| Autor: | Angèle Santenard, Emese Gazdag, Gioia Altobelli, Céline Ziegler-Birling, Olivier Poch, Laszlo Tora, Maria-Elena Torres-Padilla |
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| Přispěvatelé: | Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Peney, Maité |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Zygote
MESH: TATA-Box Binding Protein RNA polymerase II MESH: Mice Knockout Mice 0302 clinical medicine Transcription (biology) MESH: Gene Expression Regulation Developmental MESH: Animals Promoter Regions Genetic Mice Knockout 0303 health sciences Genome biology General transcription factor Gene Expression Regulation Developmental Cell Differentiation Chromatin medicine.anatomical_structure Female Germ cell Research Paper Protein Binding MESH: Cell Differentiation MESH: Oocytes MESH: Chromatin 03 medical and health sciences MESH: Gene Expression Profiling MESH: Cell Proliferation MESH: Promoter Regions Genetic Genetics medicine Animals MESH: Protein Binding MESH: Genome Transcription factor MESH: Mice Cell Proliferation 030304 developmental biology Gene Expression Profiling Promoter TATA-Box Binding Protein Molecular biology Oocytes biology.protein MESH: Zygote TATA-binding protein MESH: Female 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Genes and Development Genes and Development, Cold Spring Harbor Laboratory Press, 2009, 23 (18), pp.2210-23. ⟨10.1101/gad.535209⟩ Genes and Development, 2009, 23 (18), pp.2210-23. ⟨10.1101/gad.535209⟩ |
| ISSN: | 0890-9369 |
| DOI: | 10.1101/gad.535209⟩ |
| Popis: | Development of the germline requires consecutive differentiation events. Regulation of these has been associated with germ cell-specific and pluripotency-associated transcription factors, but the role of general transcription factors (GTFs) remains elusive. TATA-binding protein (TBP) is a GTF involved in transcription by all RNA polymerases. During ovarian folliculogenesis in mice the vertebrate-specific member of the TBP family, TBP2/TRF3, is expressed exclusively in oocytes. To determine TBP2 function in vivo, we generated TBP2-deficient mice. We found that Tbp2−/− mice are viable with no apparent phenotype. However, females lacking TBP2 are sterile due to defective folliculogenesis, altered chromatin organization, and transcriptional misregulation of key oocyte-specific genes. TBP2 binds to promoters of misregulated genes, suggesting that TBP2 directly regulates their expression. In contrast, TBP ablation in the female germline results in normal ovulation and fertilization, indicating that in these cells TBP is dispensable. We demonstrate that TBP2 is essential for the differentiation of female germ cells, and show the mutually exclusive functions of these key core promoter-binding factors, TBP and TBP2, in the mouse. |
| Databáze: | OpenAIRE |
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