F-actin interactome reveals vimentin as a key regulator of actin organization and cell mechanics in mitosis
| Autor: | Matthias Samwer, Dirk Görlich, Philippe P. Roux, Mark Petronczki, Upamali Perera, Guillaume Charras, Geneviève Lavoie, Ewa K. Paluch, Murielle P. Serres, Binh An Truong Quang |
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| Přispěvatelé: | Paluch, Ewa Kamila [0000-0003-4691-2323], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
CDK1
plectin Intermediate Filaments Vimentin macromolecular substances Biology General Biochemistry Genetics and Molecular Biology Article cell shape Cell Physiological Phenomena 03 medical and health sciences 0302 clinical medicine proteomics vimentin Chromosome Segregation Myosin Humans Cytoskeleton Intermediate filament Molecular Biology Mitosis Chromosome separation Interphase Actin 030304 developmental biology mitosis 0303 health sciences phosphorylation Cell Biology Plectin Actins Cell biology Actin Cytoskeleton cortex biology.protein mitotic rounding 030217 neurology & neurosurgery actin organization Developmental Biology HeLa Cells |
| Zdroj: | Developmental Cell |
| Popis: | Summary Most metazoan cells entering mitosis undergo characteristic rounding, which is important for accurate spindle positioning and chromosome separation. Rounding is driven by contractile tension generated by myosin motors in the sub-membranous actin cortex. Recent studies highlight that alongside myosin activity, cortical actin organization is a key regulator of cortex tension. Yet, how mitotic actin organization is controlled remains poorly understood. To address this, we characterized the F-actin interactome in spread interphase and round mitotic cells. Using super-resolution microscopy, we then screened for regulators of cortex architecture and identified the intermediate filament vimentin and the actin-vimentin linker plectin as unexpected candidates. We found that vimentin is recruited to the mitotic cortex in a plectin-dependent manner. We then showed that cortical vimentin controls actin network organization and mechanics in mitosis and is required for successful cell division in confinement. Together, our study highlights crucial interactions between cytoskeletal networks during cell division. Highlights • Comparison of the F-actin interactome in spread interphase and round mitotic cells • Proteomics identifies vimentin and plectin as key regulators of the mitotic cortex • Vimentin intermediate filaments localize under the actin cortex in mitosis • Sub-cortical vimentin regulates actin cortex organization and mechanics in mitosis Mitotic rounding, which is essential for accurate spindle positioning, is controlled by the sub-membranous cortical actin network. Using proteomics, Serres et al. identify the intermediate filament vimentin as a key regulator of the mitotic cortex. Vimentin forms a sub-cortical layer in mitosis and controls actin cortex organization and mechanics. |
| Databáze: | OpenAIRE |
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