Antioxidants, anti-inflammatory, and antidiabetic effects of the aqueous extracts from Glycine species and its bioactive compounds
| Autor: | Jeng-Shyan Deng, Hung-Jen Lin, Jui-Shu Chang, Wen-Tzu Wu, Shyh-Shyun Huang, Guan-Jhong Huang, Shan Yu Su |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Antioxidant medicine.drug_class medicine.medical_treatment Aldose reductase Genistein Plant Science Biology Anti-inflammatory 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Glycine tabacina Glycine species Daidzein biology.organism_classification 030104 developmental biology chemistry Biochemistry α-Glucosidase Polyphenol 030220 oncology & carcinogenesis Glycine Original Article |
| Zdroj: | Botanical Studies |
| ISSN: | 1817-406X |
| Popis: | Background The aim of this study was to examine the possible antioxidant, anti-inflammatory, and antidiabetic effects of the aqueous extracts from three Glycine species. In HPLC analysis, the chromatograms of three Glycine species were established. Flavonoid-related compounds might be important bioactive compounds in Glycine species. Results The results showed that the aqueous extract of Glycine tabacina (AGTa) had the strongest antioxidant activity compared with the other Glycine species extracts. We also found that AGTa had higher contents of total polyphenol compounds and flavonoids than the other extracts. We also have investigated the anti-inflammatory effects of the three Glycine species using lipopolysaccharide (LPS)-stimulated mouse macrophage (RAW264.7) ex vivo. When RAW264.7 macrophages were treated with different concentrations of three Glycine species together with LPS, a significant concentration-dependent inhibition of NO production was detected. The aqueous extract of Glycine max (AGM) had the strongest anti-inflammatory activity in comparison with the other Glycine species extracts. Western blotting revealed that three Glycine species blocked protein expression of iNOS and cyclooxygenase-2 (COX-2) in LPS-stimulated RAW264.7 macrophages, significantly. The antidiabetic activities of the three Glycine species were studied in vitro using α-glucosidase and aldose reductase (AR) inhibitory methods. AGTa had the highest inhibitory activities on α-glucosidase and aldose reductase, with IC50 of 188.1 and 126.42 μg/mL, respectively. The bioactive compounds, genistein and daidzein, had high inhibitory activities on antioxidant, anti-inflammatory, α-glucosidase and aldose reductase. Conclusions These results suggest that Glycine species might be a good resource for future development of antioxidant, anti-inflammatory and antidiabetic heath foods. |
| Databáze: | OpenAIRE |
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