Toxoplasma gondii: identification of a developmentally regulated family of genes related to SAG2
| Autor: | David J. P. Ferguson, Christine Lekutis, John C. Boothroyd |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Glycosylation
Blotting Western Molecular Sequence Data Immunology Protozoan Proteins Fluorescent Antibody Technique Antigens Protozoan Sequence alignment Biology Homology (biology) Mice chemistry.chemical_compound Antigen parasitic diseases medicine Animals Amino Acid Sequence Cloning Molecular Gene Peptide sequence Genetics Sequence Homology Amino Acid Immune Sera Toxoplasma gondii General Medicine medicine.disease biology.organism_classification Molecular biology Toxoplasmosis Infectious Diseases chemistry Multigene Family Antigens Surface Parasitology Sequence Alignment Toxoplasma |
| Zdroj: | Experimental parasitology. 96(2) |
| ISSN: | 1090-2449 0014-4894 |
| Popis: | Previous studies have shown the surface of Toxoplasma gondii to be dominated by a family of proteins closely related to SAG1. In this study, we report the existence of a second family of genes defined by homology to SAG2. The predicted amino acid sequences of these three new proteins suggests that they are all glycosylphosphatidylinositol-linked surface antigens. All three also contain N-linked glycosylation sites, although their use has yet to be demonstrated. One of these SAG2-related antigens, SAG2B, is expressed in tachyzoites with an apparent size of 23 kDa. It is distinct, however, from the previously identified P23. In contrast to SAG2B, SAG2C and SAG2D appear to be expressed exclusively on the surface of bradyzoites. Analysis of the SAG2 family shows it to have weak but significant homology to the SAG1 family. Thus, all of the sequenced surface antigens of tachyzoites and many of those of bradyzoites fall into one large superfamily that can be divided into two subgroups defined by the prototypic and highly immunogenic SAG1 and SAG2, respectively. |
| Databáze: | OpenAIRE |
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