Low light and low ammonium are key factors for guayule leaf tissue shoot organogenesis and transformation
| Autor: | Katrina Cornish, Niu Dong, Belen Montanez, Robert A. Creelman |
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| Rok vydání: | 2005 |
| Předmět: |
DNA
Bacterial Parthenium argentatum Time Factors Light Agrobacterium Organogenesis Plant Science Asteraceae chemistry.chemical_compound Transformation Genetic Murashige and Skoog medium Kanamycin Botany Glucuronidase biology Aminobutyrates fungi food and beverages General Medicine biology.organism_classification Plant Leaves Quaternary Ammonium Compounds Transformation (genetics) Glufosinate chemistry Agronomy and Crop Science Plant Shoots Explant culture Transformation efficiency |
| Zdroj: | Plant Cell Reports. 25:26-34 |
| ISSN: | 1432-203X 0721-7714 |
| DOI: | 10.1007/s00299-005-0024-2 |
| Popis: | A new method has been developed for guayule tissue culture and transformation. Guayule leaf explants have a poor survival rate when placed on normal MS medium and under normal culture room light conditions. Low light and low ammonium treatment greatly improved shoot organogenesis and transformation from leaf tissues. Using this method, a 35S promoter driven BAR gene and an ubiquitin-3 promoter driven GUS gene (with intron) have been successfully introduced into guayule. These transgenic guayule plants were resistant to the herbicide ammonium-glufosinate and were positive to GUS staining. Molecular analysis showed the expected band and signal in all GUS positive transformants. The transformation efficiency with glufosinate selection ranged from 3 to 6%. Transformation with a pBIN19-based plasmid containing a NPTII gene and then selection with kanamycin also works well using this method. The ratio of kanamycin-resistant calli to total starting explants reached 50% in some experiments. |
| Databáze: | OpenAIRE |
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