Isolation and characterization of an Escherichia coli mutant deficient in dTMP kinase activity
| Autor: | J A Fuchs, T D Daws |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Genotype
Mutant dTMP kinase Mutagenesis (molecular biology technique) medicine.disease_cause Microbiology chemistry.chemical_compound Transduction Genetic Escherichia coli Thymidine Monophosphate medicine Molecular Biology Mutation biology Phosphotransferases Temperature biology.organism_classification Molecular biology Deoxyuridine Kinetics chemistry Biochemistry Specific activity Nucleoside-Phosphate Kinase Bacteria Research Article |
| Zdroj: | Journal of Bacteriology. 157:440-444 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.157.2.440-444.1984 |
| Popis: | Escherichia coli LD0181 is sensitive to 15 micrograms of 2',3'-dideoxythymidine per ml. A derivative that was resistant to 40 micrograms of the same chemical per ml at 30 degrees C and that had lost the ability to grow on enriched medium at 42 degrees C was isolated after nitroso-guanidine mutagenesis. This mutant, TD105, produced a dTMP kinase with 25-fold lower specific activity and a 5-fold higher Km for dTMP than the parental strain. The dTMP pool in TD105 was 4.4-fold higher than in the parent. In addition to temperature sensitivity and resistance to 2',3'-dideoxythymidine, the mutant exhibited a hypersensitivity to 5-bromo-2'-deoxyuridine. All three of these phenotypes are cotransducible. The tmk gene was mapped by cotransduction to approximately 30 min on the E. coli map. |
| Databáze: | OpenAIRE |
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