Intrinsic blinking of red fluorescent proteins for super-resolution microscopy

Autor:	Konstantin A. Lukyanov, Elena V. Zagaynova, Nina G. Bozhanova, Alexander A. Moiseev, Alexander S. Mishin, Sergey A. Lukyanov, Natalia V. Klementieva, Natalie M. Mishina, Anton I. Pavlikov
Rok vydání:	2017
Předmět:	0301 basic medicine Materials science 02 engineering and technology Iterative reconstruction Catalysis 03 medical and health sciences Optics Microscopy Materials Chemistry Humans Super-resolution microscopy business.industry Metals and Alloys General Chemistry 021001 nanoscience & nanotechnology Fluorescence Surfaces Coatings and Films Electronic Optical and Magnetic Materials Luminescent Proteins 030104 developmental biology Microscopy Fluorescence Ceramics and Composites 0210 nano-technology business Algorithms HeLa Cells
Zdroj:	Chemical communications (Cambridge, England). 53(5)
ISSN:	1364-548X
Popis:	Single-molecule localization microscopy relies on either controllable photoswitching of fluorescent probes or their robust blinking. We have found that blinking of monomeric red fluorescent proteins TagRFP, TagRFP-T, and FusionRed occurs at moderate illumination power and matches well with camera acquisition speed. It allows for super-resolution image reconstruction of densely labelled structures in live cells using various algorithms.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8efc152981bb6a9fb069c9f066d4eb5f https://pubmed.ncbi.nlm.nih.gov/28044165 Zobrazit plný text záznamu