Nitric Oxide Inhibits Inflammatory Cytokine Production by Human Alveolar Macrophages
| Autor: | Serpil C. Erzurum, Lisa T. Buhrow, F. Takao Kaneko, Mani S. Kavuru, Mary Jane Thomassen, Mary J. Connors |
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| Rok vydání: | 1997 |
| Předmět: |
Lipopolysaccharides
Pulmonary and Respiratory Medicine Lipopolysaccharide Cell Survival medicine.medical_treatment Clinical Biochemistry Pharmacology Nitric Oxide Nitric oxide Proinflammatory cytokine chemistry.chemical_compound Macrophages Alveolar medicine Humans Macrophage Secretion Chemokine CCL4 Molecular Biology Chemokine CCL3 medicine.diagnostic_test business.industry Cell Biology Macrophage Inflammatory Proteins Bronchoalveolar lavage Cytokine chemistry Immunology Cytokines Tumor necrosis factor alpha business Nitroso Compounds |
| Zdroj: | American Journal of Respiratory Cell and Molecular Biology. 17:279-283 |
| ISSN: | 1535-4989 1044-1549 |
| Popis: | High levels of nitric oxide (NO) have been reported in exhaled air of asthmatic individuals. Because alveolar macrophages (AM) are major producers of cytokines, and bronchoalveolar lavage fluid (BALF) from asthmatic individuals contains increased levels of inflammatory cytokines, this study was undertaken to determine whether NO modified the production of inflammatory cytokines by human AM. AM were obtained from normal volunteers by fiberoptic bronchoscopy. Tumor necrosis factor-alpha (TNF-alpha) production stimulated by lipopolysaccharide (LPS; 0.5 microg/ml) was measured with an enzyme-linked immunosorbent assay (ELISA). NO generated from 2,2-(hydroxynitrosohydrazono)-bis-ethanamine (DETA NONOate) (0.1 to 1.0 mM) inhibited TNF-alpha secretion in a dose-dependent manner. At 1 mM DETA NONOate, mean inhibition (+/- SEM) of TNF-alpha secretion was 56 +/- 4% (P = 0.002). To determine whether this effect was cytokine specific, interleukin-1beta (IL-1beta) and macrophage inflammatory protein-1alpha (MIP-1alpha) were evaluated, and DETA NONOate was also found to inhibit both of these cytokines. Basal cytokine levels from unstimulated AM were unaffected by NO. These findings indicate that NO is a potent inhibitor of cytokine production by stimulated human AM. |
| Databáze: | OpenAIRE |
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