Exosomes derived from human umbilical cord mesenchymal stem cells protect against cisplatin-induced ovarian granulosa cell stress and apoptosis in vitro

Autor:	Dong Li, Xiuli Ju, Kun Song, Xuantao Su, Liping Sun, Lan Chao, Xiaohui Deng, Zhao Li, Shu Yao, Li Li, Beihua Kong, Jianlu Wei
Jazyk:	angličtina
Rok vydání:	2017
Předmět:	0301 basic medicine Ovarian Granulosa Cell Science Primary Cell Culture Antineoplastic Agents Apoptosis Exosomes Protective Agents Article Flow cytometry Umbilical Cord 03 medical and health sciences Bcl-2-associated X protein medicine Animals Humans Annexin A5 Rats Wistar bcl-2-Associated X Protein Multidisciplinary Granulosa Cells medicine.diagnostic_test biology Caspase 3 Mesenchymal stem cell Mesenchymal Stem Cells Flow Cytometry Microvesicles Cell biology Rats 030104 developmental biology Gene Expression Regulation Proto-Oncogene Proteins c-bcl-2 Cell culture biology.protein Medicine Female Stem cell Cisplatin Poly(ADP-ribose) Polymerases Signal Transduction
Zdroj:	Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) Scientific Reports
ISSN:	2045-2322
Popis:	Human umbilical cord mesenchymal stem cells (huMSCs) can treat primary ovarian insufficiency (POI) related to ovarian granulosa cell (OGC) apoptosis caused by cisplatin chemotherapy. Exosomes are a class of membranous vesicles with diameters of 30–200 nm that are constitutively released by eukaryotic cells. Exosomes mediate local cell-to-cell communication by transferring microRNAs and proteins. In the present study, we demonstrated the effects of exosomes derived from huMSCs (huMSC-EXOs) on a cisplatin-induced OGC model in vitro and discussed the preliminary mechanisms involved in these effects. We successfully extracted huMSC-EXOs from huMSC culture supernatant and observed the effective uptake of exosomes by cells with fluorescent staining. Using flow cytometry (with annexin-V/PI labelling), we found that huMSC-EXOs increased the number of living cells. Western blotting showed that the expression of Bcl-2 and caspase-3 were upregulated, whilst the expression of Bax, cleaved caspase-3 and cleaved PARP were downregulated to protect OGCs. These results suggest that huMSC-EXOs can be used to prevent and treat chemotherapy-induced OGC apoptosis in vitro. Therefore, this work provides insight and further evidence of stem cell function and indicates that huMSC-EXOs protect OGCs from cisplatin-induced injury in vitro.
Databáze:	OpenAIRE
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