A multistage sequencing strategy pinpoints novel candidate alleles for Emery-Dreifuss muscular dystrophy and supports gene misregulation as its pathomechanism
| Autor: | Eric C. Schirmer, E. Harris, Ulrike Schara, Manfred Wehnert, Benedikt Schoser, Peter Meinke, Alastair R.W. Kerr, Francesco Muntoni, Heike Kölbel, Volker Straub, Rafal Czapiewski, Charles R. Dixon, Jose I. de las Heras |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Candidate gene Research paper Nuclear envelope transmembrane protein primer library Medizin lcsh:Medicine Biology Genome General Biochemistry Genetics and Molecular Biology Nuclear envelope 03 medical and health sciences 0302 clinical medicine medicine Humans Emery–Dreifuss muscular dystrophy Muscular dystrophy Muscle Skeletal Gene Exome sequencing Alleles Genetics lcsh:R5-920 Genetic heterogeneity lcsh:R General Medicine medicine.disease Phenotype Muscular Dystrophy Emery-Dreifuss 3. Good health 030104 developmental biology Orphan disease Gene Expression Regulation 030220 oncology & carcinogenesis Emery-Dreifuss muscular dystrophy lcsh:Medicine (General) |
| Zdroj: | EBioMedicine, Vol 51, Iss, Pp-(2020) EBioMedicine |
| ISSN: | 2352-3964 |
| Popis: | Background: As genome-wide approaches prove difficult with genetically heterogeneous orphan diseases, we developed a new approach to identify candidate genes. We applied this to Emery-Dreifuss muscular dystrophy (EDMD), characterised by early onset contractures, slowly progressive muscular wasting, and life-threatening heart conduction disturbances with wide intra- and inter-familial clinical variability. Roughly half of EDMD patients are linked to six genes encoding nuclear envelope proteins, but the disease mechanism remains unclear because the affected proteins function in both cell mechanics and genome regulation. Methods: A primer library was generated to test for mutations in 301 genes from four categories: (I) all known EDMD-linked genes; (II) genes mutated in related muscular dystrophies; (III) candidates generated by exome sequencing in five families; (IV) functional candidates — other muscle nuclear envelope proteins functioning in mechanical/genome processes affected in EDMD. This was used to sequence 56 unlinked patients with EDMD-like phenotype. Findings: Twenty-one patients could be clearly assigned: 18 with mutations in genes of similar muscular dystrophies; 3 with previously missed mutations in EDMD-linked genes. The other categories yielded novel candidate genes, most encoding nuclear envelope proteins with functions in gene regulation. Interpretation: Our multi-pronged approach identified new disease alleles and many new candidate EDMD genes. Their known functions strongly argue the EDMD pathomechanism is from altered gene regulation and mechanotransduction due to connectivity of candidates from the nuclear envelope to the plasma membrane. This approach highlights the value of testing for related diseases using primer libraries and may be applied for other genetically heterogeneous orphan diseases. Funding: The Wellcome Trust, Muscular Dystrophy UK, Medical Research Council, European Community's Seventh Framework Programme “Integrated European –omics research project for diagnosis and therapy in rare neuromuscular and neurodegenerative diseases (NEUROMICS)”. Keywords: Emery-Dreifuss muscular dystrophy, Nuclear envelope, Nuclear envelope transmembrane protein, primer library, Orphan disease |
| Databáze: | OpenAIRE |
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