Chloroplast-localized iron superoxide dismutases FSD2 and FSD3 are functionally distinct in Arabidopsis
| Autor: | Zhong Chen, Daniel R. Gallie |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Chlorophyll
Pigments Leaves Chloroplasts Light Mutant Arabidopsis Plant Science Biochemistry chemistry.chemical_compound Superoxides Photosynthesis Materials chemistry.chemical_classification Multidisciplinary biology Plant Biochemistry Superoxide Plant Anatomy Physics Electromagnetic Radiation Eukaryota Oxides Plants Cell biology Chloroplast Chemistry Physical Sciences Hyperexpression Techniques Medicine Cellular Structures and Organelles Cellular Types Research Article Plant Cell Biology Science Materials Science Research and Analysis Methods Superoxide dismutase Plant Cells Gene Expression and Vector Techniques Nucleoid Molecular Biology Techniques Molecular Biology Gene Molecular Biology Assays and Analysis Techniques Reactive oxygen species Organic Pigments Arabidopsis Proteins Superoxide Dismutase Chemical Compounds Organisms Biology and Life Sciences Cell Biology biology.organism_classification chemistry Seedlings Mutation biology.protein |
| Zdroj: | PLoS ONE, Vol 14, Iss 7, p e0220078 (2019) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Superoxide dismutases (SODs) protect against reactive oxygen species (ROS) by detoxifying superoxide. Three types of SOD are present in plants: FeSOD, CuSOD, and MnSOD. The Arabidopsis thaliana genome contains three FeSOD genes, in which two (FSD2, and FSD3) are targeted to chloroplast thylakoids. Loss of FSD2 or FSD3 expression impairs growth and causes leaf bleaching. FSD2 and FSD3 form heterocomplexes present in chloroplast nucleoids, raising the question of whether FSD2 and FSD3 are functionally interchangeable. In this study, we examined how loss of FSD2 or FSD3 expression affects photosynthetic processes and whether overexpression of one compensates for loss of the other. Whereas loss of the cytosolic FSD1 had little effect, an fsd2 mutant exhibited increased superoxide production, reduced chlorophyll levels, lower PSII efficiency, a lower rate of CO2 assimilation, but elevated non-photochemical quenching (NPQ). In contrast, fsd3 mutants failed to survive beyond the seedling stage and overexpression of FSD2 could not rescue the seedlings. Overexpression of FSD3 in an fsd2 mutant, however, partially reversed the fsd2 mutant phenotype resulting in improved growth characteristics. Overexpression of FSD2 or FSD3, either individually or together, had little effect. These results indicate that, despite functioning as FeSODs, FSD2 and FSD3 are functionally distinct. |
| Databáze: | OpenAIRE |
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