Use of peak sharpening effects to improve the separation of chiral compounds with molecularly imprinted porous polymer layer open‐tubular capillaries
| Autor: | Milton Thomas William Hearn, Chadin Kulsing, Reinhard I Boysen, Jamil Chowdhury, Yuanzhong Yang |
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| Rok vydání: | 2017 |
| Předmět: |
Materials science
Polymers Pyridines Capillary action Clinical Biochemistry Analytical chemistry 02 engineering and technology Sharpening 01 natural sciences Biochemistry Analytical Chemistry Molecular Imprinting Capillary Electrochromatography chemistry.chemical_classification Capillary electrochromatography 010401 analytical chemistry Stereoisomerism Equipment Design Polymer 021001 nanoscience & nanotechnology 0104 chemical sciences Polymerization chemistry Racemic mixture Asparagine Enantiomer 0210 nano-technology Molecular imprinting |
| Zdroj: | ELECTROPHORESIS. 38:1179-1187 |
| ISSN: | 1522-2683 0173-0835 |
| DOI: | 10.1002/elps.201600532 |
| Popis: | This investigation demonstrates the application of a new peak sharpening technique to improve the separation of difficult-to-resolve racemic mixtures in capillary electro-chromatography. Molecularly imprinted porous layer open tubular (MIP-PLOT) capillaries, prepared by a layer-on-layer polymerization approach with Z-l-Asp-OH as the template, were selected to validate the approach. SEM revealed that the polymer film thickness can be varied by changes in both the polymer composition and the layer-on-layer regime. Capillaries made with methacrylic acid as the functional monomer could not separate the Z-Asp-OH racemate, due to weak interactions between the MIP-PLOT material and the target analytes. In contrast, MIP-PLOT capillaries prepared with 4-vinylpyridine as the functional monomer resulted in increased ionic interactions with the target analytes. Separation of the enantiomers could be enhanced when a peak zone sharpening effect was exploited through the use of specific BGE compositions and by taking advantage of eigenpeak phenomena. In this manner, the position of a sharpening zone and the peak shape of the sample analytes could be fine-tuned, so that when the sharpening zone and the target analyte co-migrated the separation of the Z-l-Asp-OH enantiomer from its d-enantiomer in a racemic mixture could be achieved under overloading conditions. |
| Databáze: | OpenAIRE |
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