Leptin: a new growth factor for the small intestine
| Autor: | Marshall Z. Schwartz, Karim Alavi, Rajeev Prasad, Darlise M. O’Connor, Vicky L. Funanage |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Leptin
Male medicine.medical_specialty Monosaccharide Transport Proteins Carbohydrate metabolism Polymerase Chain Reaction Rats Sprague-Dawley chemistry.chemical_compound Sodium-Glucose Transporter 1 Internal medicine Intestine Small medicine Animals Bovine serum albumin Intestinal Mucosa Growth Substances Infusions Intravenous Membrane Glycoproteins biology Dose-Response Relationship Drug business.industry Gene Expression Profiling Glucose Transporter Type 5 Fructose General Medicine DNA Carbohydrate Small intestine Rats Dose–response relationship Endocrinology medicine.anatomical_structure Glucose chemistry Pediatrics Perinatology and Child Health biology.protein Systemic administration Surgery business |
| Zdroj: | Journal of pediatric surgery. 37(3) |
| ISSN: | 1531-5037 |
| Popis: | Purpose: This study was designed to evaluate the potential growth factor effects of systemic administration of leptin on mucosal mass and absorptive function in normal rat intestine. Methods: Twenty male Sprague-Dawley rats underwent placement of a jugular venous catheter connected to a subcutaneous osmotic pump designed to deliver its contents at a constant rate. The rats were divided into 4 groups (n = 5 per group) based on the contents of the osmotic pump: group 1, 0.1% bovine serum albumin; group 2, leptin, 6.25 [mu ]g/kg/d; Group 3, leptin, 18.75 [mu ]g/kg/d; Group 4, leptin, 43.75 [mu ]g/kg/d. After a 14-day infusion, [ 14 C] galactose and [ 14 C] glycine absorption were determined using a closed, recirculation technique. DNA content was determined from mucosal biopsies. Total RNA was extracted from mucosal samples, reverse transcribed, and amplified via polymerase chain reaction for the following primer pairs: sodium/glucose cotransporter (SGLT-1), fructose transporter (GLUT-5), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, internal standard). Statistical analysis was performed by analysis of variance and expressed as mean [plusmn] SEM. Results: Systemic administration of increasing doses of leptin enhanced DNA content when compared with the appropriate control (group 2, 1.06 [plusmn] 0.04 [ P [lt ] .05]; group 3, 1.1 [plusmn] 0.05 [ P [lt ] .01]; and group 4, 1.07 [plusmn] 0.06 [ P [lt ] .05]. Leptin enhanced mucosal absorptive function (galactose: group 2, 2.31 [plusmn] 0.15 [ P [lt ] .01]; group 3, 2.71 [plusmn] 0.06 [ P [lt ] .01]; group 4, 2.19 [plusmn] 0.28 [ P [lt ] .05]; glycine: group 2, 2.34 [plusmn] 0.31 [ P [lt ] .05]; group 3, 3.32 [plusmn] 0.14 [ P [lt ] .01]; group 4, 3.1 [plusmn] 0.27 [ P [lt ] .01]) in the normal intestine when compared with the appropriate control animals. Also, leptin enhanced the gene expression of the carbohydrate transporters when compared with the appropriate control rats. Conclusions: These data show that systemic leptin administration enhances mucosal mass and absorptive function in normal rat intestine. Thus, leptin appears to be a growth factor for normal small intestine and may play a role in patients who acquire intestinal dysfunction. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect