Comprehensive assessment of electrospun scaffolds hemocompatibility
| Autor: | Sarka Jakubkova, Vera Jencova, Ales Saman, Jaroslava Jirousova, Petr Mikes, Jana Horakova, Tereza Svarcova, Tomas Suchy, Renata Prochazkova, Bohdana Heczkova |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Materials science
Polymers Polyesters Thrombogenicity Biocompatible Materials Bioengineering 02 engineering and technology 010402 general chemistry Hemolysis 01 natural sciences Biomaterials chemistry.chemical_compound medicine Humans Coagulation (water treatment) Platelet activation Composite material Prothrombin time Blood Cells medicine.diagnostic_test technology industry and agriculture 021001 nanoscience & nanotechnology medicine.disease 0104 chemical sciences Polyester chemistry Mechanics of Materials Polycaprolactone Microscopy Electron Scanning Prothrombin Time Partial Thromboplastin Time Collagen 0210 nano-technology Partial thromboplastin time Biomedical engineering |
| Zdroj: | Materials Science and Engineering: C. 82:330-335 |
| ISSN: | 0928-4931 |
| Popis: | Biodegradable polyesters, namely polycaprolactone (PCL) and copolymer of polylactide and polycaprolactone (PLCL) were electrospun into various fibrous structures and their hemocompatibility was evaluated in vitro. Firstly, hemolytic effect was evaluated upon incubation with diluted whole blood. The results showed that the degree of hemolysis depended on chemical composition and fibrous morphology. Electrospun polycaprolactone induced slight degree of hemolysis depending on its molecular weight and fibrous morphology; copolymer PLCL did not cause detectable hemolysis. The influence of coagulation pathways was examined by measurement of coagulation times. It was showed that intrinsic coagulation pathway assessed by activated partial thromboplastin time (APTT) was moderately accelerated after incubation with PCL and prolonged after incubation with copolymer PLCL. Extrinsic activation of coagulation tested by prothrombin time (PT) was slightly accelerated after incubation with all tested electrospun samples. Thrombogenicity assessment of fibrous samples revealed high thrombogenic properties of fibrous materials that was comparable to high degree of collagen thrombogenicity. The level of platelet activation was dependent on chemical composition and surface morphology of tested materials. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect