Plant cell growth and differentiation may involve GAP regulation of Rac activity
| Autor: | Lone Pødenphant, Carsten Poulsen, Trine Juul Jensen, Søren Borg |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Subfamily
DNA Complementary GTPase-activating protein Cellular differentiation GTPase activating protein Lotus japonicus Molecular Sequence Data Biophysics Two-hybrid screening GTPase Plasma protein binding Saccharomyces cerevisiae Cdc42/Rac interactive binding element Ligands Biochemistry GTP Phosphohydrolases Enzyme activator Structural Biology GTP-Binding Proteins Genetics Amino Acid Sequence Molecular Biology Plant Proteins Plants Medicinal biology Sequence Homology Amino Acid fungi Proteins food and beverages Fusion protein expression Cell Differentiation Fabaceae Cell Biology biology.organism_classification rac GTP-Binding Proteins Rac GTP-Binding Proteins Enzyme Activation ras GTPase-Activating Proteins Root nodule development Protein Binding Signal Transduction |
| Zdroj: | Borg, S, Pødenphant, L, Jensen, T J & Poulsen, C 1999, ' Plant cell growth and differentiation may involve GAP regulation of Rac activity ', FEBS letter, pp. 341-345 . |
| Popis: | Two Rac GTPase cDNAs, LjRac1 and LjRac2, were identified in the legume Lotus japonicus. Two-hybrid screening with dominant-constitutive mutations in the two Rac GTPases target three plant cDNAs, LjRacGAP1, LjRacGAP2 and LjRacGAP3, that encode putative GTPase activating proteins of Rho-GTPase subfamily members. Employing Rac antiserum, purified recombinant LjRac GTPases and recombinant LjRacGAP1, for ligand overlay assays, in vitro GAP affinity assays and GTPase activation, we confirmed that eukaryote Rac/RacGAP interplay is conserved in plants. In this investigation we have developed some tools that can be used to characterize the role of enhanced LjRac2 expression in developing root nodules. |
| Databáze: | OpenAIRE |
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