Regeneration of plantlets through somatic embryogenesis from root derived calli of Hibiscus sabdariffa L. (Roselle) and assessment of genetic stability by flow cytometry and ISSR analysis
| Autor: | Sinchan Adhikari, Anirban Ray, Saptarshi Konar, Tapas Kumar Bandyopadhyay, Joydeep Karmakar |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Embryology Leaves Fruit and Seed Anatomy Somatic embryogenesis Plant embryogenesis lcsh:Medicine Artificial Gene Amplification and Extension Plant Science Plant Genetics Polymerase Chain Reaction Plant Roots 01 natural sciences Plantlet chemistry.chemical_compound Spectrum Analysis Techniques Mathematical and Statistical Techniques Murashige and Skoog medium Plant Growth Regulators lcsh:Science Plant Growth and Development Principal Component Analysis Multidisciplinary Plant Anatomy Hibiscus sabdariffa Flow Cytometry Horticulture Hibiscus Spectrophotometry Embryogenesis Physical Sciences Cytophotometry Statistics (Mathematics) Plant Shoots Research Article Plant Somatic Embryogenesis Techniques Plant Development Biology Research and Analysis Methods 03 medical and health sciences Genetics Statistical Methods Molecular Biology Techniques Molecular Biology Repetitive Sequences Nucleic Acid Plant Embryo Anatomy Embryos lcsh:R fungi Cotyledons (Botany) Biology and Life Sciences Sequence Analysis DNA Culture Media 030104 developmental biology chemistry Callus Multivariate Analysis Microscopy Electron Scanning lcsh:Q Kinetin Plant Embryogenesis Mathematics Developmental Biology 010606 plant biology & botany Explant culture |
| Zdroj: | PLoS ONE, Vol 13, Iss 8, p e0202324 (2018) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Induction of somatic embryogenesis and complete plantlet regeneration from callus culture of Hibiscus sabdariffa L. var. HS4288 has been made. Leaf and root explants were cultured on Murashige and Skoog (MS) and Driver-Kuniyuki Walnut (DKW) basal media supplemented with different concentrations of synthetic auxins and cytokinins. Root explants on DKW medium supplemented with 2.26μM 2, 4-Dichlorophenoxyacetic acid (2, 4-D) and 4.65μM kinetin (KIN) induced highest percentage (70%) of embryogenic calli. Average number of globular embryos per root derived callus produced within 6 weeks of culture initiation on MS media with different plant growth regulators (PGRs) ranged from 2.27±0.12 to 8.80±0.17 and that of cotyledonary embryos ranged from 0.00 to 2.53±0.20. On DKW medium comparatively more globular embryos (2.70±0.15 to 14.53±0.23) and cotyledonary embryos (0.00 to 8.90±0.17) were produced than that of MS medium. Regeneration of complete plantlets was highest (76.67%) when embryogenic calli with mature somatic embryos were grown on DKW medium containing 2.32μM KIN and 2.22μM 6-Benzyladenine (BA). Plants were primarily hardened in humidity, temperature and light controlled chamber and finally in a greenhouse showed 70% survival ability. Different stages of somatic embryogenesis process in the root derived embryogenic calli were elaborated in detail by morphological, histological and SEM study. The data were statistically analyzed by Duncan Multiple range test (p ≤ 0.05) and Principal component analysis (PCA). Flow cytometry and Inter-simple sequence repeats (ISSR) marker analysis confirmed that there was no genetic variation within the regenerated plants. |
| Databáze: | OpenAIRE |
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