Comparative proteomic profiling of human osteoblast-derived extracellular matrices identifies proteins involved in mesenchymal stromal cell osteogenic differentiation and mineralization
| Autor: | Marta Baroncelli, Johannes P.T.M. van Leeuwen, Jeroen van de Peppel, Rodrigo D. A. M. Alves, Yik‐Yang Kan, Jeroen Demmers, Bram C. J. van der Eerden |
|---|---|
| Přispěvatelé: | Internal Medicine, Biochemistry |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Proteomics Ribosomal Proteins Stromal cell Time Factors Physiology Clinical Biochemistry Biology Mass Spectrometry Extracellular matrix 03 medical and health sciences 0302 clinical medicine Calcification Physiologic Downregulation and upregulation Osteogenesis medicine Extracellular Humans Bone regeneration Extracellular Matrix Proteins Osteoblasts Mesenchymal stem cell Osteoblast Cell Differentiation Mesenchymal Stem Cells Cell Biology Cell biology Activins 030104 developmental biology medicine.anatomical_structure Phenotype Membrane protein Immunology Electrophoresis Polyacrylamide Gel 030217 neurology & neurosurgery |
| Zdroj: | Journal of Cellular Physiology, 233(1), 387-395. Wiley-Liss Inc. |
| ISSN: | 1097-4652 0021-9541 |
| Popis: | The extracellular matrix (ECM) is a dynamic component of tissue architecture that physically supports cells and actively influences their behavior. In the context of bone regeneration, cell-secreted ECMs have become of interest as they reproduce tissue-architecture and modulate the promising properties of mesenchymal stem cells (MSCs). We have previously created an in vitro model of human osteoblast-derived devitalized ECM that was osteopromotive for MSCs. The aim of this study was to identify ECM regulatory proteins able to modulate MSC differentiation to broaden the spectrum of MSC clinical applications. To this end, we created two additional models of devitalized ECMs with different mineralization phenotypes. Our results showed that the ECM derived from osteoblast-differentiated MSCs had increased osteogenic potential compared to ECM derived from undifferentiated MSCs and non-ECM cultures. Proteomic analysis revealed that structural ECM proteins and ribosomal proteins were upregulated in the ECM from undifferentiated MSCs. A similar response profile was obtained by treating osteoblast-differentiating MSCs with Activin-A. Extracellular proteins were upregulated in Activin-A ECM, whereas mitochondrial and membrane proteins were downregulated. In summary, this study illustrates that the composition of different MSC-secreted ECMs is important to regulate the osteogenic differentiation of MSCs. These models of devitalized ECMs could be used to modulate MSC properties to regulate bone quality. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text