Preparation of a Tc-99m-labeled graft polymer and its in vitro and in vivo evaluation
| Autor: | Nefise Ülkü Karabay Yavaşoğlu, Volkan Tekin, Yasemin Parlak, Taner Türkyarar, Derya Gülcemal, Gökçen Topal, Fazilet Zumrut Biber Muftuler, Buket Bakan, Uğur Avcıbaşı, Fikriye Gül Gümüşer, Ayşegül Karadağ, Sinan Akgöl, Kevser Kuşat |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Nanopolymer
Biodistribution Proline Health Toxicology and Mutagenesis Cytotoxicity Analytical Chemistry chemistry.chemical_compound Size In vivo Prostate medicine Cytotoxic T cell Radiology Nuclear Medicine and imaging Incubation Future Spectroscopy Chromatography Graft Polymer Toxicity Chemistry Public Health Environmental and Occupational Health Magnetic Nanoparticles medicine.disease Pollution Hemolysis In vitro Tc-99m medicine.anatomical_structure Nuclear Energy and Engineering Graft polymer Adsorption Therapy Delivery Radiolabeling |
| Popis: | The aim of this study is the synthesis of a novel Tc-99m-labeld graft polymer and the biological evaluation of its in vitro and in vivo properties. To this end, a L-proline-graft-poly(HEMA) was prepared and labeled with Tc-99m. The radiochemical yield of approximately the Tc-99m-labeled compound amounted to 97 +/- 2.3%. The cytotoxicity test revealed no cytotoxic effect after a 24- and 48-h incubation. The results of the hemolysis test showed that hemolysis was non-toxic with an effect level of less than 2%. Subsequently, the biodistribution in healthy rats was determined. High accumulation of the polymer was observed in the pancreas, thyroid and prostate. Manisa Celal Bayar University Coordination Unit of Scientific Research Projects (BAP) [2017-016] The authors are thankful for the financial support from the Manisa Celal Bayar University Coordination Unit of Scientific Research Projects (BAP) (Project number: 2017-016). We thank to Buket Ate for the technical assistance during the animal experiments. We also thank Norma R. de Yagcier and Mahdi Rajabimovahed for reviewing our study as native English speakers. |
| Databáze: | OpenAIRE |
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