Next-Generation Survey Sequencing and the Molecular Organization of Wheat Chromosome 6B
| Autor: | Hiroaki Sakai, Hiroyuki Kanamori, Hana Šimková, Takashi R. Endo, Takashi Matsumoto, Katsuyuki Hayakawa, Jianzhong Wu, Ritsuko Onuki, Takeshi Itoh, Tsuyoshi Tanaka, Yasunari Ogihara, Jaroslav Doležel, Giri Prasad Joshi, Shuhei Nasuda, Hirokazu Handa, Fuminori Kobayashi |
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| Rok vydání: | 2013 |
| Předmět: |
Cancer genome sequencing
Genetics RNA Untranslated chromosome 6B Shotgun sequencing Chromosome Mapping High-Throughput Nucleotide Sequencing food and beverages General Medicine Genome project Full Papers Biology Chromosomes Plant DNA sequencing Deep sequencing genome sequencing wheat next-generation sequencing Transcriptome Molecular Biology Genome size Triticum Illumina dye sequencing Exome sequencing |
| Zdroj: | DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes |
| ISSN: | 1756-1663 1340-2838 |
| Popis: | Common wheat (Triticum aestivum L.) is one of the most important cereals in the world. To improve wheat quality and productivity, the genomic sequence of wheat must be determined. The large genome size (∼17 Gb/1 C) and the hexaploid status of wheat have hampered the genome sequencing of wheat. However, flow sorting of individual chromosomes has allowed us to purify and separately shotgun-sequence a pair of telocentric chromosomes. Here, we describe a result from the survey sequencing of wheat chromosome 6B (914 Mb/1 C) using massively parallel 454 pyrosequencing. From the 4.94 and 5.51 Gb shotgun sequence data from the two chromosome arms of 6BS and 6BL, 235 and 273 Mb sequences were assembled to cover ∼55.6 and 54.9% of the total genomic regions, respectively. Repetitive sequences composed 77 and 86% of the assembled sequences on 6BS and 6BL, respectively. Within the assembled sequences, we predicted a total of 4798 non-repetitive gene loci with the evidence of expression from the wheat transcriptome data. The numbers and chromosomal distribution patterns of the genes for tRNAs and microRNAs in wheat 6B were investigated, and the results suggested a significant involvement of DNA transposon diffusion in the evolution of these non-protein-coding RNA genes. A comparative analysis of the genomic sequences of wheat 6B and monocot plants clearly indicated the evolutionary conservation of gene contents. コムギのゲノム配列の概要解読に成功 -コムギの新品種開発の加速化に期待-. 京都大学プレスリリース. 2014-07-24. |
| Databáze: | OpenAIRE |
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