Protective effects of erythropoietin against acute lung injury in a rat model of acute necrotizing pancreatitis
| Autor: | Ali Ugur Emre, Mustafa Comert, Handan Ankarali, Kemal Karakaya, Oktay Irkorucu, Guldeniz Karadeniz Cakmak, Gürkan Kertiş, Burak Bahadir, Hakan Balbaloglu, Bulent Hamdi Ucan, Ishak Ozel Tekin, Oge Tascilar, Serefden Acikgoz |
|---|---|
| Přispěvatelé: | Zonguldak Bülent Ecevit Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Male
medicine.medical_specialty acute pancreatitis Neutrophils Pleural effusion Lung injury Proinflammatory cytokine Lipid peroxidation chemistry.chemical_compound Malondialdehyde Internal medicine medicine cytokine Animals Rats Wistar Lung Respiratory Distress Syndrome Taurodeoxycholic Acid Interleukin-6 Pancreatitis Acute Necrotizing business.industry Body Weight Gastroenterology General Medicine respiratory system acute respiratory distress syndrome medicine.disease respiratory tract diseases Rats Lipoproteins LDL Pleural Effusion Pulmonary Alveoli Disease Models Animal Basic Research Endocrinology medicine.anatomical_structure chemistry acute lung injury Erythropoietin Amylases Immunology Interleukin-2 Pancreatitis erythropoietin business medicine.drug |
| Popis: | WOS: 000251840200007 PubMed: 18069756 AIM: To investigate the effect of exogenous erythropoietin (EPO) administration on acute lung injury (ALI) in an experimental model of sodium taurodeoxycholate-induced acute necrotizing pancreatitis (ANP). METHODS: Forty-seven male Wistar albino rats were randomly divided into 7 groups: sham group (n = 5), 3 ANP groups (n = 7 each) and 3 EPO groups (n = 7 each). ANP was induced by retrograde infusion of 5% sodium taurodeoxycholate into the common bile duct. Rats in EPO groups received 1000 U/kg intramuscular EPO immediately after induction of ANP Rats in ANP groups were given 1 mL normal saline instead. All animals were sacrificed at postoperative 24 h, 48 h and 72 h. Serum amilase, IL-2, IL-6 and lung tissue malondialdehyde (MDA) were measured. Pleural effusion volume and lung/body weight (LW/BW) ratios were calculated. Tissue levels of TNF-alpha, IL-2 and IL-6 were screened immunohistochemically. Additionally, ox-LDL accumulation was assessed with immune-fluorescent staining. Histopathological alterations in the lungs were also scored. RESULTS: The mean pleural effusion volume, calculated LW/BW ratio, serum IL-6 and lung tissue MDA levels were significantly lower in EPO groups than in ANP groups. No statistically significant difference was observed in either serum or tissue values of IL-2 among the groups. The level of tumor necrosis factor-alpha (TNF-alpha) and IL-6 and accumulation of ox-LDL were evident in the lung tissues of ANP groups when compared to EPO groups, particularly at 72 h. Histopathological evaluation confirmed the improvement in lung injury parameters after exogenous EPO administration, particularly, at 48 h and 72 h. CONCLUSION: EPO administration leads to a significant decrease in ALI parameters by inhibiting polymorphonuclear leukocyte (PMNL) accumulation, decreasing the levels of proinflammatory cytokines in circulation, preserving microvascular endothelial cell integrity and reducing oxidative stress-associated lipid peroxidation and therefore, can be regarded as a cytoprotective agent in ANP-induced ALI. (c) 2007 WJG. All rights reserved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|