Depletion of intracellular glutathione and increased lipid peroxidation mediate cytotoxicity of hematite nanoparticles in MRC-5 cells
| Autor: | Andreea Iren Serban, Anca Dinischiotu, S.N. Petrache, Mihaela Radu, Maria Cristina Munteanu, Diana Dinu, Anca Hermenean, Cornelia Sima |
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| Rok vydání: | 2010 |
| Předmět: |
Antioxidant
Cell Survival medicine.medical_treatment Glutathione reductase Pharmacology GPX4 Ferric Compounds General Biochemistry Genetics and Molecular Biology Cell Line Superoxide dismutase Lipid peroxidation chemistry.chemical_compound medicine Humans chemistry.chemical_classification Glutathione Peroxidase biology Superoxide Dismutase Glutathione peroxidase Glutathione Malondialdehyde Catalase Oxidative Stress Glutathione Reductase chemistry Biochemistry biology.protein Nanoparticles Lipid Peroxidation |
| Zdroj: | Acta biochimica Polonica. 57(3) |
| ISSN: | 1734-154X |
| Popis: | Particles generated from numerous anthropogenic and/or natural sources, such as crystalline α-Fe₂O₃ nanoparticles, have the potential to damage lung cells. In our study we investigated the effects of these nanoparticles (12.5 µg/ml) on lipid peroxidation and the antioxidative system in MRC-5 lung fibroblast cells following exposure for 24, 48 or 72h. Exposure to α-Fe₂O₃ nanoparticles increased lipid peroxidation by 81%, 189% and 110% after 24, 48 and 72h, respectively. Conversely, the reduced glutathione concentration decreased by 23.2% and 51.4% after 48 and 72h of treatment, respectively. In addition, an augmentation of the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione transferase and glutathione reductase within the interval between 48-72h was noticed. Taking into account that the reduced glutathione level decreased and the malondialdehyde level, a lipid peroxidation product, remained highly increased up to 72h of exposure, it would appear that the MRC-5 antioxidant defense mechanisms did not efficiently counteract the oxidative stress induced by exposure to hematite nanoparticles. |
| Databáze: | OpenAIRE |
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