Electrochemical aptamer-based microsensor for real-time monitoring of adenosine in vivo
| Autor: | Di Zhang, Xiangwen Meng, Jiajia Ma, Yuxin Fang, Yi Guo, Jian Zhang, Zhifang Xu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
Adenosine Aptamer Guanosine Metal Nanoparticles 02 engineering and technology Biosensing Techniques 01 natural sciences Biochemistry Analytical Chemistry Nanoclusters chemistry.chemical_compound Limit of Detection medicine Environmental Chemistry Animals Rats Wistar Electrodes Spectroscopy chemistry.chemical_classification Detection limit Chemistry Biomolecule 010401 analytical chemistry Nucleic Acid Hybridization DNA Electrochemical Techniques Aptamers Nucleotide 021001 nanoscience & nanotechnology Uridine 0104 chemical sciences Methylene Blue Electrode Biophysics Graphite Indicators and Reagents Gold 0210 nano-technology DNA Probes medicine.drug |
| Zdroj: | Analytica chimica acta. 1076 |
| ISSN: | 1873-4324 |
| Popis: | In this work, an implantable and minimally invasive micro-aptasensor for adenosine monitoring in vivo, based on flexible integrated electrodes, was developed. Firstly the sensor was made by the modification of a needle-type electrode with reduced graphene oxide and gold nanoclusters (rGO-AuNCs) using two-step electrodeposition. Secondly Sulfhydryl-terminated capture probe (ssDNA1) was immobilized on rGO-AuNCs modified electrode surface by self-assembly, and then it was hybridized with adenosine aptamer (ssDNA2). Lastly methylene blue (MB) as an electrochemical indicator was adsorbed on the aptamer through specific interaction of MB with guanine base. The peak current of MB decreased linearly with increasing adenosine concentration due to the formation of aptamer-adenosine complex and displacement of the aptamer from the modified electrode surface. The sensor showed a low detection limit of 0.1 nM with signal-to-noise ratio equal to 3 as well as a wide linear response range (0.1 nM-1 mM) in vitro. Also, a high selectivity was demonstrated for adenosine in relation to uridine, guanosine, and cytidine. Experiments in vivo demonstrated fast responses for a range of adenosine concentrations. This work demonstrates a promising path for implantable devices for the determination of biomolecules in vivo, thus allowing for health tests, detection of infectious diseases, and other medical conditions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect