The Conserved Intron Binding Protein EMB-4 Plays Differential Roles in Germline Small RNA Pathways of C. elegans
| Autor: | Monica Z. Wu, Amena Nabih, Christopher J. Wedeles, Julia A. Sobotka, Katarzyna M. Tyc, Julie M. Claycomb |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Small RNA Biology General Biochemistry Genetics and Molecular Biology Germline Transcriptome 03 medical and health sciences 0302 clinical medicine Animals RasiRNA RNA Messenger Caenorhabditis elegans Caenorhabditis elegans Proteins Molecular Biology Genetics Messenger RNA Adult Germline Stem Cells Intron Nuclear Proteins Cell Biology Argonaute RNA silencing 030104 developmental biology Argonaute Proteins RNA Interference 030217 neurology & neurosurgery Protein Binding Developmental Biology |
| Zdroj: | Developmental Cell. 42:256-270.e6 |
| ISSN: | 1534-5807 |
| Popis: | Summary Proper regulation of the germline transcriptome is essential for fertility. In C. elegans , germline homeostasis hinges on a complex repertoire of both silencing and activating small RNA pathways, along with RNA processing. However, our understanding of how fundamental RNA processing steps intersect with small RNA machineries in the germline remains limited. Here, we link the conserved intron binding protein, EMB-4/AQR/IBP160, to the CSR-1 and HRDE-1 nuclear 22G-RNA pathways in the C. elegans germline. Loss of emb-4 leads to distinct alterations in CSR-1- versus HRDE-1-associated small RNA and mRNA transcriptomes. Our transcriptome-wide analysis shows that EMB-4 is enriched along pre-mRNAs of nearly 8,000 transcripts. While EMB-4 complexes are enriched for both intronic and exonic sequences of HRDE-1 targets, CSR-1 pathway targets are enriched for intronic, but not exonic, sequences. These data suggest that EMB-4 could contribute to a molecular signature that distinguishes the targets of these two germline small RNA pathways. |
| Databáze: | OpenAIRE |
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