Iodine-124 labelled annexin-V as a potential radiotracer to study apoptosis using positron emission tomography
| Autor: | Patricia M Price, Lisa Bouchier-Hayes, Eric O. Aboagye, Sajinder K. Luthra, Frank Brady, Matthias Glaser, Seamus J. Martin, O. Clyde Hutchinson, David R. Collingridge |
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| Rok vydání: | 2002 |
| Předmět: |
chemistry.chemical_element
Apoptosis HL-60 Cells Iodine Benzoates Iodine Radioisotopes Tosyl Compounds Drug Stability Annexin medicine Tumor Cells Cultured Humans Annexin A5 Radioactive Tracers Radiation Chromatography medicine.diagnostic_test Trimethyltin Compounds Radiochemistry Chloramines Fast protein liquid chromatography Biological activity Hydrogen-Ion Concentration chemistry Positron emission tomography Reagent Isotope Labeling Camptothecin Electrophoresis Polyacrylamide Gel Radiopharmaceuticals medicine.drug Chromatography Liquid Tomography Emission-Computed |
| Zdroj: | Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine. 58(1) |
| ISSN: | 0969-8043 |
| Popis: | Annexin-V is a calcium-dependent protein that binds with high affinity to phosphaditylserine exposed during apoptosis. The aim of this study was to radiolabel annexin-V with iodine-124 for use as a potential probe of apoptosis by positron emission tomography. Annexin-V was radioiodinated directly using the cyclotron-produced positron emitter iodine-124 by the chloramine-T (CAT) method and indirectly by the pre-labelled reagent N-succinimidyl 3-[124I]iodobenzoate ([124I]m-SIB). Some reaction parameters of the CAT method such as reaction time and pH were optimised to give radiochemical yields of 22.3 +/- 2.6%(n = 3, gel-filtration). After incubation with [124I]m-SIB, radiolabelled annexin-V was obtained in 14% and 25% yield by FPLC and gel-filtration, respectively. The radiochemical purities from direct and indirect labelling were 97.7 +/- 1.0%(n = 3) and 96.7 +/- 2.1%(n = 3), respectively. The new radiotracers could be stored for up to four days without significant de-iodination. The biological activity of radiolabelled annexin-V was tested in control and camptothecin-treated (i.e. apoptotic) human leukaemic HL60 cells. A significantly higher (21%) binding in treated cells was observed with [125I]m-SIB-annexin-V. The binding of [125I]m-SIB labelled annexin-V to camptothecin treated cells was blocked (68%) by a 100-fold excess of unlabelled annexin-V. |
| Databáze: | OpenAIRE |
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