Coelenterazine Analogue with Human Serum Albumin-Specific Bioluminescence
| Autor: | Ryo Nishihara, Kazuki Niwa, Ryoji Kurita, Tatsunosuke Tomita |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pharmacology
Luminescence Chromatography Alkylation Chemistry Organic Chemistry Imidazoles Biomedical Engineering Pharmaceutical Science Serum Albumin Human Bioengineering Human serum albumin Serum samples Luciferin chemistry.chemical_compound Pyrazines Coelenterazine medicine Benzyl group Humans Bioluminescence Biotechnology medicine.drug |
| Zdroj: | Bioconjugate Chemistry. 31:2679-2684 |
| ISSN: | 1520-4812 1043-1802 |
| DOI: | 10.1021/acs.bioconjchem.0c00536 |
| Popis: | A synthetic luciferin comprising an imidazopyrazinone core, named HuLumino1, was designed to generate specific bioluminescence with human serum albumin (HSA) in real serum samples. HuLumino1 was developed by attaching a methoxy-terminated alkyl chain to C-6 of coelenterazine and by eliminating a benzyl group at C-8. HSA levels were quantified within 5% error margins of an enzyme-linked immunosorbent assay without the need for any sample pretreatments because of the high specificity of HuLumino1. |
| Databáze: | OpenAIRE |
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