Comparison of conventional and supported liquid extraction methods for the determination of sitagliptin and simvastatin in rat plasma by LC–ESI–MS/MS
| Autor: | P. Sita Devi, B. Ramesh, V.U.M. Sarma, S.R. Bijargi, Nemali Manjula |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Analyte
Simvastatin Lc esi ms ms Pharmaceutical Science Pharmacy Analytical Chemistry Pharmacokinetics Drug Discovery Electrochemistry medicine Supported liquid extraction Sitagliptin Solid phase extraction Spectroscopy Medicine(all) Chromatography Biochemistry Genetics and Molecular Biology(all) Chemistry Extraction (chemistry) lcsh:RM1-950 lcsh:Therapeutics. Pharmacology Rat plasma Extraction methods Original Article medicine.drug |
| Zdroj: | Journal of Pharmaceutical Analysis Journal of Pharmaceutical Analysis, Vol 5, Iss 3, Pp 161-168 (2015) |
| ISSN: | 2214-0883 2095-1779 |
| Popis: | Three extraction methods were compared for their efficiency to analyze sitagliptin and simvastatin in rat plasma by LCâMS/MS, including (1) liquidâliquid extraction (LLE), (2) solid phase extraction (SPE) and (3) supported liquid extraction (SLE). Comparison of recoveries of analytes with different extraction methods revealed that SLE was the best extraction method. The detection was facilitated with ion trap-mass spectrometer by multiple reactions monitoring (MRM) in a positive ion mode with ESI. The transitions monitored were m/z 441.1â325.2 for simvastatin, 408.2â235.1 for sitagliptin and 278.1â260.1 for the IS. The lower limit of quantification (LLOQ) was 0.2Â ng/mL for sitagliptin and 0.1Â ng/mL for simvastatin. The effective SLE offers enhanced chromatographic selectivity, thus facilitating the potential utility of the method for routine analysis of biological samples along with pharmacokinetic studies. Keywords: Rat plasma, Simvastatin, Sitagliptin, Supported liquid extraction |
| Databáze: | OpenAIRE |
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