TAL effectors: tools for DNA Targeting
| Autor: | Radek Jankele, Petr Svoboda |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Genetics
Transcription activator-like effector nuclease Xanthomonas FokI Nucleotides Effector Nucleic acid sequence General Medicine Biology Biochemistry Zinc finger nuclease DNA-Binding Proteins TALEN Genome editing TAL effector Tandem repeat RNA editing DNA editing Papers TALE RNA Editing ZFN Molecular Biology Transcription Factors |
| Zdroj: | Briefings in Functional Genomics |
| ISSN: | 2041-2657 2041-2649 |
| DOI: | 10.1093/bfgp/elu013 |
| Popis: | Xanthomonas phytopathogenic bacteria produce unique transcription activator-like effector (TALE) proteins that recognize and activate specific plant promoters through a set of tandem repeats. A unique TALE-DNA-binding code uses two polymorphic amino acids in each repeat to mediate recognition of specific nucleotides. The order of repeats determines effector's specificity toward the cognate nucleotide sequence of the sense DNA strand. Artificially designed TALE-DNA-binding domains fused to nuclease or activation and repressor domains provide an outstanding toolbox for targeted gene editing and gene regulation in research, biotechnology and gene therapy. Gene editing with custom-designed TALE nucleases (TALENs) extends the repertoire of targeted genome modifications across a broad spectrum of organisms ranging from plants and insect to mammals. |
| Databáze: | OpenAIRE |
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