Utility of Basophil Activation Test for monitoring the acquisition of clinical tolerance after oral desensitization to cow’s milk: Pilot study
| Autor: | Domenico Schiavino, Eleonora Nucera, Giovanni Gasbarrini, Giampiero Patriarca, Anna Giulia Ricci, Alessia Di Rienzo, Angela Rizzi, Lucilla Pascolini, Manuela Ferraironi, Valentina Pecora, Alessandro Buonomo, Arianna Aruanno, Simona Mezzacappa |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
biology
CD63 business.industry medicine.medical_treatment Settore MED/09 - MEDICINA INTERNA Gastroenterology food and beverages Milk allergy Original Articles Basophil Immunoglobulin E medicine.disease Basophil activation medicine.anatomical_structure Oncology Food allergy Casein Immunology biology.protein medicine Basophil Activation Test business Desensitization (medicine) |
| Popis: | Objective: The quantification of basophil activation by flow cytometry is a useful tool for the assessment of immediate-type responses to food allergens and the prediction of clinical tolerance in food allergy patients. The aim of this study is to investigate how the analysis of allergen-induced CD63 up-regulation by flow cytometry can be effective in monitoring the acquisition of clinical tolerance by specific oral desensitization in food allergy. To our knowledge, this is the first study to examine this topic. Materials and methods: Three male patients affected by cow’s milk allergy underwent successful oral desensitization to cow’s milk. In order to monitor the acquired clinical tolerance that occurred after treatment, we performed laboratory tests for total and specific IgE, specific IgG4 and the Basophil Activation Test (BAT) both at baseline and at the end of the desensitization protocol. Results: Using a fluorescent enzyme immunoassay, the comparison of specific cow’s milk antibodies before and after treatment showed a decrease of specific IgE levels, without reaching normal values, and an increase of specific IgG4 levels. A complete suppression of cow’s milk proteins (a-lactoalbumin, b-lactoglobulin and casein) induced CD63 regulation was observed in all three reported cases. Conclusions: Using flow cytometry, food allergen-specific basophil responses could be monitored in order to identify an acquired tolerance induced by desensitization treatment. Although further studies are needed to develop this important new topic, it was interesting to note that the BAT seemed to be more sensitive and characterized by a close correlation with clinical tolerance. |
| Databáze: | OpenAIRE |
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