Genome characterization of a novel porcine bocavirus
| Autor: | Can-ping Huang, Zhao-jun Duan, Jie-mei Yu, Wan-Zhu Yang, Jin-song Li, Wei-xia Cheng |
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| Rok vydání: | 2012 |
| Předmět: |
Nucleotide Sequence Difference
Human Bocavirus Swine Sequence analysis Molecular Sequence Data Sequence Homology Genome Viral Biology Healthy Piglet Genome Protein Structure Secondary DNA sequencing Bocavirus Viral Proteins chemistry.chemical_compound Phylogenetics Terminology as Topic Virology Animals Cluster Analysis Protein secondary structure Phylogeny Genetics Whole genome sequencing Phylogenetic tree Sequence Analysis DNA General Medicine Pathogenic Avian Influenza H5N1 Virus chemistry DNA Viral Original Article DNA |
| Zdroj: | Archives of Virology |
| ISSN: | 1432-8798 0304-8608 |
| Popis: | Using a high-throughput DNA sequencing method, one DNA sequence (contig01006), suspected to belong to a novel porcine bocavirus (PBoV), was found with a high rate of detection (19.6 %) in fecal samples from healthy piglets. Moreover, a novel PBoV (tentatively named PBoV3C) with a nearly complete genome sequence (5235 bp) was identified. PBoV3C exhibits typical genome characteristics of bocaviruses and shows the highest genomic sequence identity (78 % to 81 %) to PBoV3A/B (PBoV3/4-UK) and PBoV3D/E (PBoV3/4-HK), respectively. Phylogenetic and recombination analysis indicated high diversity, prevalence and complexity among the PBoVs. The phospholipase A2 (PLA2) site of VP1 and the secondary structure of VP2 of PBoV3C were also analyzed. Additionally, we propose a uniform method of PBoV nomenclature based on the VP1 gene. |
| Databáze: | OpenAIRE |
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