Gene Expression Analysis of the IPEC-J2 Cell Line: A Simple Model for the Inflammation-Sensitive Preterm Intestine

Autor:	Ann Cathrine Findal Støy, Per T. Sangild, Mette V. Østergaard, Peter M. H. Heegaard, Kerstin Skovgaard
Rok vydání:	2013
Předmět:	Article Subject Biology Intestinal epithelium Molecular biology Small intestine Immune system medicine.anatomical_structure Cell culture Immunology Gene expression medicine CXCL10 Colostrum Interleukin 8
Zdroj:	Støy, A C F, Heegaard, P M H, Sangild, P T, Østergaard, M V & Skovgaard, K 2013, ' Gene Expression Analysis of the IPEC-J2 Cell Line: A Simple Model for the Inflammation-Sensitive Preterm Intestine ', ISRN Zoology, vol. 2013, 980651 . https://doi.org/10.1155/2013/980651 Støy, A C F, Heegaard, P M H, Sangild, P T, Østergaard, M V & Skovgaard, K 2013, ' Gene Expression Analysis of the IPEC-J2 Cell Line: A Simple Model for the Inflammation-Sensitive Preterm Intestine ' ISRN Genomics, vol. 2013, 980651 . https://doi.org/10.1155/2013/980651
ISSN:	2314-4637
Popis:	The IPEC-J2 cell line was studied as a simple model for investigating responses of the newborn intestinal epithelium to diets. Especially, the small intestine of immature newborns is sensitive to diet-induced inflammation. We investigated gene expression of epithelial- and immune response-related genes in IPEC-J2 cells stimulated for 2 h with milk formula (CELL-FORM), colostrum (CELL-COLOS), or growth medium (CELL-CONTR) and in distal small intestinal tissue samples from preterm pigs fed milk formula (PIG-FORM) or colostrum (PIG-COLOS). High throughput quantitative PCR analysis of 48 genes revealed the expression of 22 genes in IPEC-J2 cells and 31 genes in intestinal samples. Principal component analysis (PCA) discriminated the gene expression profile of IPEC-J2 cells from that of intestinal samples. The expression profile of intestinal tissue was separated by PCA into 2 groups according to diet, whereas no diet-dependent grouping was seen for IPEC-J2 cells. Expression differences between PIG-FORM and PIG-COLOS were found for DEFB1, CXCL10, IL1RN, and ALPI, while IL8 was upregulated in CELL-FORM compared with CELL-CONTR. These differences, between IPEC-J2 cells and intestinal tissue from preterm pigs, both used as models for the newborn intestine, underline that caution must be exercised prior to analysis and interpretation of diet-induced effects on gene expression.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ece0448afc0b14bdce189ca91faf9bf https://doi.org/10.1155/2013/980651 Zobrazit plný text záznamu