LLC-PK1 cells: cloning of phenotypically stable subpopulations

Autor: Dominique Belin, Lelio Orci, Jean-Dominique Vassalli, Annelise Isabelle Wohlwend
Jazyk: angličtina
Rok vydání: 1986
Předmět:
Calcitonin
medicine.medical_specialty
Cholera Toxin
Cholera Toxin/pharmacology
Physiology
Vasopressins
Swine
Clone (cell biology)
RNA
Messenger/metabolism
Vasopressins/pharmacology
Biology
Kidney
Kidney/ cytology/drug effects/physiology
Epithelium
Cell Line
Basal (phylogenetics)
Plasminogen Activators
Mice
Internal medicine
medicine
Calcitonin binding
Animals
Plasminogen Activators/metabolism
RNA
Messenger
Calcitonin/metabolism/pharmacology
Clone Cells/cytology/physiology
Cloning
ddc:616
Neoplasms
Experimental/etiology
Mice
Inbred BALB C
Epithelium/physiology
urogenital system
Electric Conductivity
Cell Biology
Neoplasms
Experimental
Molecular biology
Urokinase-Type Plasminogen Activator
Clone Cells
Molecular Weight
Endocrinology
medicine.anatomical_structure
Phenotype
Cell culture
Electrophoresis
Polyacrylamide Gel
Urokinase-Type Plasminogen Activator/genetics/metabolism
Plasminogen activator
Zdroj: American Journal of Physiology, Vol. 250, No 5 Pt 1 (1986) pp. C682-687
ISSN: 0002-9513
Popis: The LLC-PK1 pig kidney-derived cell line is morphologically and functionally heterogeneous. We have clonally derived three sublines that differ in their response to calcitonin and in their ability to form domes. The three clones were analyzed for their basal and hormonally induced plasminogen activator production. In contrast to the D + Sc clone, in which calcitonin induced a greater than 100-fold increase in plasminogen activator synthesis, the D + Rc clone did not respond to the hormone; this was related to a deficiency of the cells in calcitonin binding. Transepithelial electrical resistance measurements revealed a direct correlation with the capacity of the cells to form domes; in one of the isolated clones (D-), the lack of dome formation coincided with a low electrical resistance; the D + Sc clone, in which all single cell-derived colonies formed domes, showed a higher electrical resistance than that developed by the original cell line. Thus the LLC-PK1 clones provide a useful in vitro model for the study of epithelial properties.
Databáze: OpenAIRE
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