Phenotypic Characterization of Arabidopsis Ascorbate and Glutathione Deficient Mutants under Abiotic Stresses
| Autor: | Dong-Phuong Tra, Minh Thi Thanh Hoang, Thi Phuong Thao Dang, Phuong Ngo Diem Quach, Thanh Nguyen Chu, Mai Thi Anh Doan, Thuong Nguyen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
leaf area seed germination root architecture Photosynthesis 01 natural sciences cad2-1 lcsh:Agriculture 03 medical and health sciences chemistry.chemical_compound abiotic stress tolerance vtc2-4 030304 developmental biology Abiotic component 0303 health sciences photosynthesis Abiotic stress Chemistry Lateral root fungi lcsh:S food and beverages ascorbate (AsA) Glutathione Ascorbic acid glutathione (GSH) vtc5-2 Horticulture Germination Shoot Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Agronomy Volume 11 Issue 4 Agronomy, Vol 11, Iss 764, p 764 (2021) |
| ISSN: | 2073-4395 |
| DOI: | 10.3390/agronomy11040764 |
| Popis: | Ascorbic acid (AsA) and glutathione (GSH) are considered important factors to protect plants against abiotic stress. To investigate whether altered endogenous GSH and AsA affect seed germination, plant performance and the abiotic stress tolerance, GSH deficient mutant cad2-1 and AsA-deficient mutants (vtc2-4 and vtc5-2) were phenotypically characterized for their seed germination, shoot growth, photosynthetic activity and root architecture under abiotic stresses. The cad2-1, vtc2-4 and vtc5-2 mutants showed a decrease in osmotic and salt stress tolerance, in sensitivity to ABA during seed germination, and in plant performance under severe abiotic stresses. GSH deficiency in the cad2-1 plants affected plant growth and root development in plants exposed to strong drought, oxidative and heavy metal stress conditions. Plants with lower GSH did not show an increased sensitivity to strong salt stress (100 mM NaCl). In contrast, the mutants with lower AsA enhanced salt stress tolerance in the long-term exposures to strong salt stress since they showed larger leaf areas, longer primary roots and more lateral root numbers. Limitations on AsA or GSH synthesis had no effect on photosynthesis in plants exposed to long-term strong salt or drought stresses, whereas they effected on photosynthesis of mutants exposed to CdCl2. Taken together, the current study suggests that AsA and GSH are important for seed germination, root architecture, shoot growth and plant performance in response to different abiotic stresses, and their functions are dependent on the stress-inducing agents and the stress levels. |
| Databáze: | OpenAIRE |
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