Gelatin methacryloyl as environment for chondrocytes and cell delivery to superficial cartilage defects
| Autor: | Sara Žigon-Branc, Katja Hölzl, Marian Fürsatz, Marica Markovic, Anne Kleiner, Hakan Göcerler, Sylvia Nürnberger, Stefan Baudis, Pauschitz Andreas, Barbara Schädl, Sandra Van Vlierberghe, Aleksandr Ovsianikov, Jasper Van Hoorick, Claudia Gahleitner, Heinz Redl |
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| Rok vydání: | 2021 |
| Předmět: |
Cell type
food.ingredient chondrocytes Biomedical Engineering biocompatible materials Medicine (miscellaneous) Osteoarthritis Gelatin MESENCHYMAL STEM-CELLS Biomaterials Chondrocytes food GROWTH-FACTOR RELEASE CHONDROGENESIS stem cells EXTRACELLULAR-MATRIX medicine Humans Viability assay cartilage gelatin methacryloyl REPAIR Tissue Engineering Chemistry HYDROGEL Cartilage Biology and Life Sciences Hydrogels ARTICULAR-CARTILAGE Cell delivery Biocompatible material medicine.disease osteoarthritis medicine.anatomical_structure STROMAL CELLS Methacrylates KNEE Stem cell Biomedical engineering |
| Zdroj: | JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE |
| ISSN: | 1932-7005 1932-6254 |
| Popis: | Cartilage damage typically starts at its surface, either due to wear or trauma. Treatment of these superficial defects is important in preventing degradation and osteoarthritis (OA). Biomaterials currently used for deep cartilage defects lack appropriate properties for this application. Therefore, we investigated photo-crosslinked methacrylamide-modified gelatin (gelMA) as a candidate for treatment of surface defects. It allows for liquid application, filling of surface defects and forming a protective layer after UV-crosslinking, thereby keeping therapeutic cells in place. GelMA and photo-initiator (Li-TPO) concentration were optimized for application as a carrier to create a favourable environment for human articular chondrocytes (hAC). Primary hAC were used in passages 3 and 5, encapsulated into two different gelMA concentrations (7.5 wt% (soft) and 10 wt% (stiff)) and cultivated for 3 weeks with TGF-β3 (0, 1 and 10 ng/mL). Higher TGF-β3 concentrations induced spherical cell morphology independent of gelMA stiffness, while low TGF-β3 concentrations only induced rounded morphology in stiff gelMA. Gene expression did not vary across gel stiffnesses. As a functional model gelMA was loaded with two different cell types (hAC and/or human adipose-derived stem cells (ASC/TERT1) and applied to human osteochondral osteoarthritic plugs. GelMA attached to the cartilage, smoothened the surface and retained cells in place. Resistance against shear forces was tested using a tribometer, simulating normal human gait and revealing maintained cell viability. In conclusion gelMA is a versatile, biocompatible material with good bonding capabilities to cartilage matrix, allowing sealing and smoothening of superficial cartilage defects while simultaneously delivering therapeutic cells for tissue regeneration. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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