A specific radioimmunoassay for osteocalcin with advantageous species crossreactivity
| Autor: | Richard E. Grindeland, Cathy E. Brautigam, Patricia Patterson-Allen, Paul X. Callahan, C. Willet Asling |
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| Rok vydání: | 1982 |
| Předmět: |
musculoskeletal diseases
medicine.medical_specialty Osteocalcin Radioimmunoassay Biophysics Connective tissue Kidney Biochemistry Bone and Bones Mice Dogs Species Specificity Antibody Specificity Internal medicine biology.animal medicine Animals Humans Horses Molecular Biology chemistry.chemical_classification biology musculoskeletal neural and ocular physiology Calcium-Binding Proteins Horse Rats Inbred Strains Haplorhini Cell Biology musculoskeletal system Rats Amino acid medicine.anatomical_structure Endocrinology chemistry Cats biology.protein Cattle Antibody Papio Baboon |
| Zdroj: | Analytical Biochemistry. 120:1-7 |
| ISSN: | 0003-2697 |
| Popis: | The specificities of immunoassays to rat and bovine osteocalcin are examined. Extracts of noncalcified tissues and tissue fractions are unreactive to the antibody, with the exception of the kidney, in which the reactive component appears to be identical with osteocalcin by gel filtration and dose dilution analysis. The assays, developed against protein isolated from bone, are also demonstrated to be reactive to the native protein (bone in situ) and to osteocalcin in serum. The assays are sensitive to less than 50 pg osteocalcin. Intra- and interassay coefficients of variation are less than 6.8%. The bovine antibody crossreacts with human, horse, monkey, baboon, and cat osteocalcin, while the rat antibody crossreacts with dog and mouse. |
| Databáze: | OpenAIRE |
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