The expression of the carboxyl ester lipase gene in pancreas and pancreatic adenocarcinomas
| Autor: | Winfried Padberg, Martina Sauter, Susanne Aberle, Karin Klingel, Nikolaus Blin, Folker Ernst Franke, Annette Greschniok, Reinhard Reuss |
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| Rok vydání: | 2006 |
| Předmět: |
Cancer Research
Pancreatic disease In situ hybridization Biology Adenocarcinoma Gene Expression Regulation Enzymologic Pancreatic cancer Gene expression medicine Biomarkers Tumor Humans RNA Messenger Lipase Pancreas In Situ Hybridization Carcinoma Acinar Cell Reverse Transcriptase Polymerase Chain Reaction Liver Neoplasms RNA Probes Suicide gene medicine.disease Molecular biology Adenocarcinoma Mucinous Reverse transcription polymerase chain reaction Pancreatic Neoplasms medicine.anatomical_structure Oncology Biochemistry Pancreatitis biology.protein Carcinoma Pancreatic Ductal |
| Zdroj: | International journal of oncology. 29(3) |
| ISSN: | 1019-6439 |
| Popis: | Since pancreatic cancer is an aggressive and often incurable malignancy, we investigated if the carboxyl ester lipase gene (CEL) is specifically expressed in pancreatic tissues and its promoter can be used for a specific suicide gene approach. Twenty-five tumor samples, 24 samples of normal pancreatic tissue and control tissues from other organs were examined by radioactive in situ hybridization (ISH) to localize CEL mRNA. Two carcinoma samples and 6 permanent cell lines were examined by reverse transcriptase-polymerase chain reaction (RT-PCR). By ISH, we verified a strong CEL gene expression in acinar cells of the normal pancreas. A minor expression was noted in a single sample of acinar cell carcinoma and adenocarcinomas did not show any expression. By RT-PCR, no specific expression in both tested adenocarcinomas was observed. In summary, these results show that, contrary to notable expression of carboxyl ester lipase in acinar cells of normal pancreatic tissue, this lipase is not significantly active in pancreatic adenocarcinomas and thus not an apt genetic marker for diagnostic or therapeutic approaches. |
| Databáze: | OpenAIRE |
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