Structure and biological properties of exopolysaccharide isolated from Citrobacter freundii
| Autor: | Anish Nag, Anutosh Patra, Soumitra Mondal, Bikas R. Pati, Nandan Bhattacharyya, Kalyani Khanra, Indranil Choudhuri, Gajendra Nath Maity, Prasenjit Maity |
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| Rok vydání: | 2021 |
| Předmět: |
Magnetic Resonance Spectroscopy
Antioxidant Cell Survival medicine.medical_treatment Size-exclusion chromatography Mannose Antineoplastic Agents 02 engineering and technology Biochemistry Antioxidants 03 medical and health sciences chemistry.chemical_compound Structural Biology medicine Humans Cytotoxicity Sugar Molecular Biology Cell Proliferation 030304 developmental biology 0303 health sciences Chromatography biology Hydrolysis Polysaccharides Bacterial General Medicine 021001 nanoscience & nanotechnology biology.organism_classification In vitro Citrobacter freundii Molecular Weight Carbohydrate Sequence chemistry Yield (chemistry) Chromatography Gel 0210 nano-technology HeLa Cells |
| Zdroj: | International Journal of Biological Macromolecules. 168:537-549 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2020.12.063 |
| Popis: | This study aimed to investigate the molecular characterization, antioxidant activity in vitro, cytotoxicity study of an exopolysaccharide isolated from Citrobacter freundii. Firstly, the culture conditions were standardized by the Design of experiments (DoE) based approach, and the final yield of thecrude exopolysaccharide was optimized at 2568 ± 169 mg L−1. One large fraction of exopolysaccharide was obtained from the culture filtrate by size exclusion chromatography and molecular characteristics were studied. A new mannose rich exopolysaccharide (Fraction-I) with average molecular weight ~ 1.34 × 105 Da was isolated. The sugar analysis showed the presence of mannose and glucose in a molar ratio of nearly 7:2 respectively. The structure of the repeating unit in the exopolysaccharide was determined through chemical and 1D/2D- NMR experiments as: Download : Download high-res image (71KB) Download : Download full-size image Finally, the antioxidant activity, and the cytotoxicity of the exopolysaccharide were investigated and the relationship with molecular properties was discussed as well. |
| Databáze: | OpenAIRE |
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