The essential roles of Mps1 in spermatogenesis and fertility in mice
| Autor: | Qiang Fang, Feng Sun, Xiao-Mei Yang, Jian-Feng Chang, Xue-Lin Chen, Tian-Zeng Sun, Ya-Bin Li, Chen-Xin Yang, Lei Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Cancer Research Cell division Somatic cell Immunology Mitosis Biology Protein Serine-Threonine Kinases Article 03 medical and health sciences Cellular and Molecular Neuroscience Mice Prophase Gonocyte Meiosis Spermatocytes Conditional gene knockout medicine Animals Spermatogenesis Infertility Male 030304 developmental biology Mice Knockout 0303 health sciences QH573-671 030302 biochemistry & molecular biology Cell Biology Spermatogonia Cell biology Mice Inbred C57BL medicine.anatomical_structure Fertility Cytology Germ cell |
| Zdroj: | Cell Death & Disease Cell Death and Disease, Vol 12, Iss 6, Pp 1-13 (2021) |
| ISSN: | 2041-4889 |
| Popis: | Monopolar spindle 1 (MPS1), which plays a critical role in somatic mitosis, has also been revealed to be essential for meiosis I in oocytes. Spermatogenesis is an important process involving successive mitosis and meiosis, but the function of MPS1 in spermatogenesis remains unclear. Here, we generated Mps1 conditional knockout mice and found that Ddx4-cre-driven loss of Mps1 in male mice resulted in depletion of undifferentiated spermatogonial cells and subsequently of differentiated spermatogonia and spermatocytes. In addition, Stra8-cre-driven ablation of Mps1 in male mice led to germ cell loss and fertility reduction. Spermatocytes lacking Mps1 have blocked at the zygotene-to-pachytene transition in the prophase of meiosis I, which may be due to decreased H2B ubiquitination level mediated by MDM2. And the expression of many meiotic genes was decreased, while that of apoptotic genes was increased. Moreover, we also detected increased apoptosis in spermatocytes with Mps1 knockout, which may have been the reason why germ cells were lost. Taken together, our findings indicate that MPS1 is required for mitosis of gonocytes and spermatogonia, differentiation of undifferentiated spermatogonia, and progression of meiosis I in spermatocytes. |
| Databáze: | OpenAIRE |
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